放射防护剂2-巯基乙胺对recA诱导的抑制作用。

Inhibition of recA induction by the radioprotector 2-mercaptoethylamine.

作者信息

Näslund M, Kolman A, Ehrenberg L

机构信息

Department of Radiobiology, Stockholm University, Sweden.

出版信息

Mutat Res. 1992 Jul;282(3):203-7. doi: 10.1016/0165-7992(92)90096-z.

DOI:10.1016/0165-7992(92)90096-z

PMID:1378554

Abstract

Our earlier finding that the radioprotective action of 2-mercaptoethylamine (MEA) is counteracted by ascorbate suggests a biochemical mechanism of action, which is supported by observations that MEA is not radioprotective in Rec- E. coli strains. In this study we show that MEA inhibits the induction of the recA gene by UV- or gamma-irradiation or by nalidixic acid in Escherichia coli strain GE94, which contains a recA-lacZ fusion. This effect, which may be counteracted by cysteine, indicates that in general MEA inhibits the induction of SOS functions.

摘要

我们早期的研究发现，抗坏血酸盐会抵消2-巯基乙胺（MEA）的辐射防护作用，这提示了一种生化作用机制，而MEA在Rec - 大肠杆菌菌株中无辐射防护作用的观察结果也支持了这一机制。在本研究中，我们表明MEA可抑制大肠杆菌GE94菌株中紫外线或γ射线照射或萘啶酸诱导的recA基因，该菌株含有recA - lacZ融合基因。这种作用可能会被半胱氨酸抵消，这表明一般情况下MEA会抑制SOS功能的诱导。