一种在体外第一次有丝分裂时获取纯淋巴细胞群体的简单便捷方法。

A simple and convenient method for gaining pure populations of lymphocytes at the first mitotic division in vitro.

作者信息

Chen D Q, Zhang C Y

机构信息

Laboratory of Industrial Hygiene, Ministry of Public Health, Deshengmenwai, Beijing, China.

出版信息

Mutat Res. 1992 Jul;282(3):227-9. doi: 10.1016/0165-7992(92)90100-v.

DOI:10.1016/0165-7992(92)90100-v

PMID:1378558

Abstract

This paper describes a simple method for obtaining pure populations of human lymphocytes at the first in vitro mitotic division (M-1) by continuous treatment with colchicine or colcemid. When colchicine (7.2 x 10(-8) M) or colcemid (4.8 x 10(-8) M) was added to cultures 0-24 h after initiation, cultures harvested at 54 h had more than 99.9% of mitotic cells in the first metaphase (M-1). We showed that not only more analyzable M-1 cells may be obtained, but staining for sister-chromatid differentiation may be avoided.

摘要

本文描述了一种通过用秋水仙碱或秋水仙酰胺连续处理来在首次体外有丝分裂期（M-1）获得纯人淋巴细胞群体的简单方法。当在培养开始后0至24小时向培养物中加入秋水仙碱（7.2×10⁻⁸ M）或秋水仙酰胺（4.8×10⁻⁸ M）时，在54小时收获的培养物中，超过99.9%的有丝分裂细胞处于第一次中期（M-1）。我们表明，不仅可以获得更多可分析的M-1细胞，而且可以避免姐妹染色单体分化染色。