YAMAMOTO R, SADLER W W, ADLER H E, STEWART G F
Appl Microbiol. 1961 Jul;9(4):337-42. doi: 10.1128/am.9.4.337-342.1961.
Strains of Clostridium perfringens capable of producing heat-resistant spores, characteristic of the food-poisoning types, were not recovered in a random survey of feces and livers of market poultry. Favorable growth response with a known food-poisoning strain indicated that the media and methods employed were adequate. Spores produced in vitro from this strain survived at 100 C for several hours. Animal feeding experiments with this strain showed that heat-resistant spores (surviving for 1 hr at 100 C) could be readily demonstrated 24 hr after oral instillation of vegetative cells in mouse feces, but not in chicken feces. One experiment suggests that this strain might adapt to the environment of the intestinal tract of chickens, but not all of the spores recovered were as heat resistant as those of the parent culture.
在对市售家禽的粪便和肝脏进行的随机调查中,未发现能够产生耐热孢子(这是食物中毒类型的特征)的产气荚膜梭菌菌株。已知食物中毒菌株呈现出良好的生长反应,这表明所采用的培养基和方法是合适的。该菌株在体外产生的孢子在100℃下可存活数小时。用该菌株进行的动物喂养实验表明,在给小鼠口服营养细胞24小时后,可在小鼠粪便中轻易检测到耐热孢子(在100℃下存活1小时),但在鸡粪便中则未检测到。一项实验表明,该菌株可能适应鸡肠道的环境,但回收的所有孢子并不都像亲代培养物的孢子那样耐热。