Weiss K F, Strong D H, Groom R A
Appl Microbiol. 1966 Jul;14(4):479-85. doi: 10.1128/am.14.4.479-485.1966.
Spores and vegetative cells of Clostridium perfringens, in combination with meat or starch paste, sterile culture filtrates, lecithinase, and phosphorylcholine, were administered to mice and rhesus monkeys in an attempt both to evaluate the animals as test agents and, if possible, to elucidate the active factors producing food-poisoning symptoms caused by this organsim. Some of the preparations were administered to the monkeys by stomach tube; others, in gelatin capsules which were treated with formaldehyde so that the release of their contents was delayed and presumably reached the intestines of the animals. Any changes in intestinal passage times and in consistency of stools of the animals were observed, and the counts of C. perfringens in the feces of the monkeys previous and subsequent to treatment were recorded. The results obtained were inconclusive. Diarrhea occurred only relatively infrequently in both species, regardless of the substance fed or the mode of administration. The changes in intestinal passage times were not great, although in the monkeys there appeared to be a slight trend toward reduction as the magnitude of the bacterial load increased. Phosphorylcholine appeared to have little, if any, effect in reducing intestinal passage time of mice or monkeys. No procedures explored in these experiments could be said to be satisfactory as a means of animal assay for food poisoning strains of C. perfringens since typical symptoms did not appear with regularity.
将产气荚膜梭菌的孢子和营养细胞与肉或淀粉糊、无菌培养滤液、卵磷脂酶和磷酸胆碱相结合，给小鼠和恒河猴注射，旨在评估这些动物作为试验对象的情况，并尽可能阐明导致该微生物引起食物中毒症状的活性因子。部分制剂通过胃管给猴子注射；其他制剂则制成明胶胶囊，胶囊经过甲醛处理，以使内容物延迟释放，并推测能到达动物肠道。观察动物肠道通过时间和粪便稠度的任何变化，并记录治疗前后猴子粪便中产气荚膜梭菌的数量。所得结果尚无定论。无论喂食何种物质或采用何种给药方式，腹泻在这两个物种中都只是相对较少发生。肠道通过时间的变化不大，尽管在猴子中，随着细菌负荷量的增加，似乎有轻微的缩短趋势。磷酸胆碱似乎对缩短小鼠或猴子的肠道通过时间几乎没有作用（如果有作用的话）。由于典型症状并未规律出现，这些实验中探索的任何方法都不能说是用于产气荚膜梭菌食物中毒菌株动物检测的令人满意的手段。
