Matsumura T, Itoh H, Watanabe N, Oda Y, Tanaka M, Namba M, Kono N, Matsuyama T, Komatsu R, Matsuzawa Y
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1992 Jul 15;186(1):503-8. doi: 10.1016/s0006-291x(05)80836-8.
We previously reported that GLP-1(7-36)amide had glucagonostatic action as well as insulinotropic action in the perfused rat pancreas. In this study, we examined the effect of GLP-1(7-36)amide on glucagon secretion and cAMP concentration in glucagon-secreting cell line, In-R1-G9. GLP-1(7-36)amide (1nM) significantly suppressed glucagon secretion and decreased cAMP concentration in the cells. GLP-1(1-37) did not affect glucagon secretion. It is suggested that inhibitory effect of GLP-1(7-36)amide on glucagon secretion is at least partly mediated by adenylate cyclase system.
我们之前报道过，胰高血糖素样肽-1(7-36)酰胺在灌注大鼠胰腺中具有抑制胰高血糖素的作用以及促胰岛素分泌作用。在本研究中，我们检测了胰高血糖素样肽-1(7-36)酰胺对胰高血糖素分泌细胞系In-R1-G9中胰高血糖素分泌和环磷酸腺苷（cAMP）浓度的影响。胰高血糖素样肽-1(7-36)酰胺（1纳摩尔）显著抑制了细胞中胰高血糖素的分泌，并降低了cAMP浓度。胰高血糖素样肽-1(1-37)对胰高血糖素分泌没有影响。提示胰高血糖素样肽-1(7-36)酰胺对胰高血糖素分泌的抑制作用至少部分是由腺苷酸环化酶系统介导的。
