Harry G J, Morell P, Bouldin T W
Brain and Development Research Center, University of North Carolina, Chapel Hill.
J Neurosci Res. 1992 Mar;31(3):554-60. doi: 10.1002/jnr.490310320.
The right L5 dorsal root ganglion of adult rats exposed to acrylamide (40 mg/kg body weight/day for nine consecutive days) was injected with either [3H]methionine or [3H]glucosamine. After allowing incorporation into macromolecules and axonal transport to proceed for 5 hr, the distribution of radioactivity in cross sections and longitudinal sections of sciatic nerve was determined by autoradiography. Control and treated animals showed no difference in distribution of label within the sciatic nerve with respect to rapidly transported proteins labelled with [3H]methionine. In control animals the distribution of rapidly transported glycoproteins labelled with [3H]glucosamine was similar to that found for [3H]methionine-labelled proteins. In contrast, acrylamide-exposed rats had a very different distribution of labelled glycoproteins; there was a marked paucity of label in the myelinated axons. We interpret this result as indicating that acrylamide preferentially inhibits glycosylation or axonal transport of glycoproteins in neurons bearing myelinated axons.
给连续九天暴露于丙烯酰胺（40毫克/千克体重/天）的成年大鼠的右侧L5背根神经节注射[3H]甲硫氨酸或[3H]葡糖胺。在让其掺入大分子并进行轴突运输5小时后，通过放射自显影术确定坐骨神经横切面和纵切面中放射性的分布。对照动物和处理过的动物在坐骨神经内，对于用[3H]甲硫氨酸标记的快速运输蛋白而言，标记分布没有差异。在对照动物中，用[3H]葡糖胺标记的快速运输糖蛋白的分布与用[3H]甲硫氨酸标记的蛋白的分布相似。相反，暴露于丙烯酰胺的大鼠标记糖蛋白的分布非常不同；有髓轴突中的标记明显缺乏。我们将这一结果解释为表明丙烯酰胺优先抑制有髓轴突神经元中糖蛋白的糖基化或轴突运输。
