Gingras D, Bendayan M
Department of Anatomy, Université de Montréal, Québec, Canada.
Pancreas. 1992;7(4):477-85. doi: 10.1097/00006676-199207000-00010.
By applying morphometrical and quantitative double immunocytochemical techniques, differences in size and in amylase and chymotrypsinogen contents were found among pancreatic zymogen granules. These differences were present in granules of peri-insular and tele-insular acinar cells, the peri-insular ones displaying higher numbers of granules of smaller sizes. No correlation was found among enzyme contents in individual granules, nor was there a correlation between enzyme content and granule size. The results suggest that each individual secretory granule is formed in an independent way and that each enzyme is processed and packaged into granules independently. The differences among granules may be associated with nonparallel secretion, since this phenomenon has been reported in the intracellular processing of secretory enzymes. This hypothesis, however, remains to be demonstrated.
通过应用形态测量和定量双重免疫细胞化学技术，发现胰腺酶原颗粒在大小以及淀粉酶和胰凝乳蛋白酶原含量方面存在差异。这些差异存在于胰岛周围和远侧胰岛腺泡细胞的颗粒中，胰岛周围的颗粒呈现出数量更多、尺寸更小的特点。在单个颗粒中的酶含量之间未发现相关性，酶含量与颗粒大小之间也没有相关性。结果表明，每个单独的分泌颗粒是以独立的方式形成的，并且每种酶都是独立加工并包装到颗粒中的。颗粒之间的差异可能与非平行分泌有关，因为这种现象在分泌酶的细胞内加工过程中已有报道。然而，这一假设仍有待证实。
