使用中和单克隆抗体通过竞争放射免疫分析（RIA）对口蹄疫病毒C3雷森德亚型进行分析。

Foot-and-mouth disease virus C3 Resende subtype analysed by means of competition RIA using neutralizing monoclonal antibodies.

作者信息

Valcic M A, Panjevic D J

机构信息

Department of Infectious diseases of animals and bees, Veterinary Faculty, University of Belgrade, Yugoslavia.

出版信息

Zentralbl Veterinarmed B. 1992 May;39(3):193-200. doi: 10.1111/j.1439-0450.1992.tb01158.x.

DOI:10.1111/j.1439-0450.1992.tb01158.x

PMID:1379398

Abstract

Foot-and-mouth disease virus (FMDV) was analysed using 30 monoclonal antibodies (MAbs) obtained from Balb/c mice immunized with FMDV C3 Resende (C3R) subtype 7 and 14 days before fusion No. 15 and 16 respectively. Fourteen MAbs were neutralizing and by means of competition radioimmuno assay it was possible to classify them into four groups. The first group consisted of MAbs specific for three sequential and three conformational epitopes. The second group consisted of MAbs specific for two conformational and for one sequential epitope. The third and the fourth groups consisted only of one MAb each, being specific for conformationally and one sequentially dependent epitope, respectively.

摘要

使用从分别在第15次和第16次融合前14天用口蹄疫病毒C3雷森德（C3R）亚型7免疫的Balb/c小鼠获得的30种单克隆抗体（MAb）对口蹄疫病毒（FMDV）进行了分析。14种MAb具有中和作用，通过竞争放射免疫测定法可以将它们分为四组。第一组由针对三个连续表位和三个构象表位的MAb组成。第二组由针对两个构象表位和一个连续表位的MAb组成。第三组和第四组每组仅由一种MAb组成，分别针对构象依赖性表位和一个连续依赖性表位。