Histochemistry of the human hair follicle with consideration of anagen phases I to VI.

The hair follicle is composed of different epithelial layers under participation of mesenchymal and nerval factors. The present study is an attempt to localize differentiation and functional markers in the human hair follicle during anagen phases I to VI. Monoclonal antibody K 8.12 against keratins 13 and 16 showed an increasing reactivity with certain types of the follicle epithelia during anagen I to VI. Ki67 was expressed within the innermost layer of the outer hair root sheath. Scattered Ki67-positive matrix cells could be additionally identified during anagen V and VI but were absent in anagen I to IV. During anagen Merkel cells became more abundant in the bulbar area. Neuropeptide-like immunoreactivity expressed by bulbar (especially matrix) cells were evenly seen during the early anagen I and II. The findings are in favour of a neurohumoral modulatory role during anagen phases accompanied by an increase of expression of certain proliferation-associated antigens like keratin 16 and Ki67 among the complex epithelia of human hair follicles.