Accili D, Menghi G, Bondi A M, Scocco P
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
Acta Histochem. 1992;92(2):196-206. doi: 10.1016/S0065-1281(11)80081-4.
Sugar specific lectins (PNA, RCA I, LPA, SBA, DBA, GSA IB4, GSA II, WGA, LTA, UEA I, Con A, LCA) with and without prior selective glycosidase digestion (sialidase, alpha-fucosidase, alpha-mannosidase, beta-N-acetylglucosaminidase, alpha- and beta-galactosidase, beta-glucosidase) were used in order to investigate the distribution of native accessible carbohydrates and obtain information dealing with the composition of terminal disaccharides within glycoconjugates present in acinar compartments and ductal segments of mammalian (mouse, rat, hare, and rabbit) parotid glands. Glycoconjugates containing variable amounts of mannose, glucose, N-acetylgalactosamine and N-acetylglucosamine were present in the parotid glands of all species. However, these carbohydrate chains exhibited a different composition of terminal sequences within each type of gland. For example, sialylated components having the terminal dimers sialic acid-galactose and sialic acid-N-acetylgalactosamine were found in all acinar cells, whereas fucoglycoconjugates with terminal disaccharide fucose-galactose were localized in the rat striated ducts and hare acinar cells. The terminal sequence alpha-galactose-beta-galactose was demonstrated in the mouse acinar cells. Finally, glycoconjugates characterized by the terminal dimer beta-galactose-N-acetylgalactosamine were demonstrated in the mouse acinar and ductal cells and the rat ductal ones. Thus, present findings outlined and further confirmed the possibility to elucidate the oligosaccharide structure in situ using lectin histochemistry combined with enzymatic degradation.
使用带有和不带有预先选择性糖苷酶消化（唾液酸酶、α-岩藻糖苷酶、α-甘露糖苷酶、β-N-乙酰氨基葡萄糖苷酶、α-和β-半乳糖苷酶、β-葡萄糖苷酶）的糖特异性凝集素（PNA、RCA I、LPA、SBA、DBA、GSA IB4、GSA II、WGA、LTA、UEA I、Con A、LCA），以研究天然可及碳水化合物的分布，并获取有关哺乳动物（小鼠、大鼠、野兔和兔子）腮腺腺泡区和导管段中存在的糖缀合物内末端二糖组成的信息。所有物种的腮腺中都存在含有不同量甘露糖、葡萄糖、N-乙酰半乳糖胺和N-乙酰氨基葡萄糖的糖缀合物。然而，这些碳水化合物链在每种腺体类型中表现出不同的末端序列组成。例如，在所有腺泡细胞中都发现了具有末端二聚体唾液酸-半乳糖和唾液酸-N-乙酰半乳糖胺的唾液酸化成分，而具有末端二糖岩藻糖-半乳糖的岩藻糖糖缀合物则定位于大鼠纹状管和野兔腺泡细胞中。在小鼠腺泡细胞中证实了末端序列α-半乳糖-β-半乳糖。最后，在小鼠腺泡和导管细胞以及大鼠导管细胞中证实了以末端二聚体β-半乳糖-N-乙酰半乳糖胺为特征的糖缀合物。因此，目前的研究结果概述并进一步证实了使用凝集素组织化学结合酶促降解原位阐明寡糖结构的可能性。
