Labate M, Desantis S, Corriero A
Istituto di Zoologia e Anatomia Comparata dell'Università di Bari, Italy.
Eur J Histochem. 1997;41(1):47-56.
The epididymal ductuli efferentes of the lizard Podarcis sicula campestris De Betta are lined with simple, columnar, nonciliated and ciliated cells. The use of lectin histochemistry has provided information about changes of sugars associated with glycoconjugates of epithelial cells and intraluminal spermatozoa which are conveyed from the longitudinal canal to the cranial region of the ductus epididymis. Epithelial cells exhibited residues of alpha-D-mannose, N-acetylglucosamine, and beta-D-galactose-(1-4)-N-acetylglucosamine as revealed with lectins Con A, WGA and RCA120, respectively, throughout the sexual cycle. An increase of RCA120 staining was observed on microvilli of nonciliated cells and in the cytoplasm of ciliated cells during the reproductive period. However, during the following refractory period, when the organ was in regression, there was a decreased staining with Con A on microvilli and the absorbent surface of nonciliated cells, with WGA in nonciliated cells and the cytoplasm of ciliated cells, and with RCA120 on microvilli and the cytoplasm of both cell types. Terminal alpha-D-galactose residues were increasingly stained from autumn up to the reproductive period with BS I-B4 on microvilli, the absorbent surface and cilia, whereas they were entirely lacking during the refractory period. UEA I revealed alpha-L-fucose residues on the absorbent surface of nonciliated cells during the abortive and reproductive periods, increasing in the latter period when cilia also expressed this sugar. Terminal alpha/beta-D-N-acetylgalactosamine was evidenced with SBA on the absorbent surface of nonciliated cells during the reproductive period. The terminal beta-D-galactose-(1-3)-N-acetylgalactosamine dimer was never found with PNA, whereas O- and N-linked sialoglycoconjugates were present only during the reproductive period. The spermatozoa head exhibited N-linked glycans with high-mannose content, and beta-D-galactose-(1-4)-N-acetylglucosamine as well as O- and N-linked sialoglycoconjugates throughout the year. During the reproductive period, oligosaccharides with alpha-D-mannose residues increased, and oligosaccharides with terminal alpha-D-galactose, alpha-L-fucose and sialic acid-N-acetylgalactosamine dimer were also present. Unlike spermatozoa of seminiferous tubules, the spermatozoa head of the lizard epididymal ductuli efferentes exhibited seasonal variability in the lectin binding pattern which may be related to time-dependent changes in the glycoconjugate profiles of epithelial cells.
意大利壁蜥（Podarcis sicula campestris De Betta）附睾输出小管由单层柱状的非纤毛细胞和纤毛细胞构成。凝集素组织化学方法的应用为我们提供了一些信息，这些信息与上皮细胞和管腔内精子的糖缀合物中糖类的变化有关，这些糖类物质从纵管运输至附睾管的头端区域。在整个性周期中，上皮细胞分别通过凝集素Con A、WGA和RCA120显示出α-D-甘露糖、N-乙酰葡糖胺和β-D-半乳糖-（1-4）-N-乙酰葡糖胺的残基。在繁殖期，观察到非纤毛细胞微绒毛和纤毛细胞胞质中RCA120染色增加。然而，在随后的不应期，当器官退化时，Con A在非纤毛细胞微绒毛和吸收表面的染色减少，WGA在非纤毛细胞和纤毛细胞胞质中的染色减少，RCA120在两种细胞类型的微绒毛和胞质中的染色减少。从秋季到繁殖期，终末α-D-半乳糖残基在微绒毛、吸收表面和纤毛上用BS I-B4染色逐渐增加，而在不应期则完全没有。UEA I显示在流产期和繁殖期非纤毛细胞吸收表面有α-L-岩藻糖残基，在繁殖期纤毛也表达这种糖时，该残基增加。在繁殖期，用SBA在非纤毛细胞吸收表面证实有终末α/β-D-N-乙酰半乳糖胺。用PNA从未发现终末β-D-半乳糖-（1-3）-N-乙酰半乳糖胺二聚体，而O-和N-连接的唾液酸糖缀合物仅在繁殖期存在。精子头部全年都有高甘露糖含量的N-连接聚糖、β-D-半乳糖-（1-4）-N-乙酰葡糖胺以及O-和N-连接的唾液酸糖缀合物。在繁殖期，含有α-D-甘露糖残基的寡糖增加，同时也存在含有终末α-D-半乳糖、α-L-岩藻糖和唾液酸-N-乙酰半乳糖胺二聚体的寡糖。与生精小管的精子不同，意大利壁蜥附睾输出小管的精子头部在凝集素结合模式上表现出季节性变化，这可能与上皮细胞糖缀合物谱随时间的变化有关。
