系统性硬化症患者双阴性α/β T细胞的T细胞受体Vβ谱系
T cell receptor V beta repertoire of double-negative alpha/beta T cells in patients with systemic sclerosis.
作者信息
Sakamoto A, Sumida T, Maeda T, Itoh M, Asai T, Takahashi H, Yoshida S, Koike T, Tomioka H, Yoshida S
机构信息
Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
出版信息
Arthritis Rheum. 1992 Aug;35(8):944-8. doi: 10.1002/art.1780350815.
OBJECTIVE
To analyze the T cell receptor V beta gene on double-negative (DN) alpha/beta T cells, which are increased in number, on peripheral blood lymphocytes (PBL) from patients with systemic sclerosis (SSc).
METHODS
The DN alpha/beta T cells were sorted by flow cytometry from PBL obtained from 3 patients with SSc. The V beta repertoire was analyzed by polymerase chain reaction.
RESULTS
Only 1 or 2 V beta genes (V beta 5/7, 5, or 17) were predominantly expressed on DN alpha/beta T cells from these 3 patients.
CONCLUSION
The V beta repertoire on DN alpha/beta T cells in PBL from patients with SSc is rather restricted.
目的
分析系统性硬化症（SSc）患者外周血淋巴细胞（PBL）上数量增加的双阴性（DN）α/βT细胞的T细胞受体Vβ基因。
方法
通过流式细胞术从3例SSc患者获取的PBL中分选DNα/βT细胞。采用聚合酶链反应分析Vβ谱。
结果
这3例患者的DNα/βT细胞上主要仅表达1种或2种Vβ基因（Vβ5/7、5或17）。
结论
SSc患者PBL中DNα/βT细胞的Vβ谱相当受限。
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