体外操作、维持和克隆单个哺乳动物细胞的新方法。
New method for manipulation, maintenance, and cloning of single mammalian cells in vitro.
作者信息
Aronson M, Kessel R W
出版信息
Science. 1960 May 6;131(3410):1376-7. doi: 10.1126/science.131.3410.1376.
Abstract
Individual mammalian cells can be isolated with the aid of easily handled glass beads to which the cells have become attached. Procedures for the preparation of cell cultures on beads, and for the recognition and manipulation of beads carrying single monocytes, HeLa cells, and Detroit-98 cells are described. Data obtained with Brucella infected monocytes, illustrating the efficiency of the method, are presented.
摘要
借助易于操作的玻璃珠可分离单个哺乳动物细胞，细胞会附着在玻璃珠上。本文描述了在玻璃珠上制备细胞培养物以及识别和操作携带单个单核细胞、HeLa细胞和底特律98细胞的玻璃珠的方法。还展示了用布鲁氏菌感染的单核细胞获得的数据，以说明该方法的有效性。
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