LOCKART R Z, EAGLE H
Science. 1959 Jan 30;129(3344):252-4. doi: 10.1126/science.129.3344.252.
A minimal growth medium supplemented with dialyzed serum, which sufficed for the propagation of a wide variety of human cell strains in heavily inoculated monolayer and suspension cultures, did not permit the regular or optimal growth of small numbers of HeLa, HeLa S3, conjunctiva, or KB cells deriving from suspension cultures. At threshold concentrations of serum, the plating efficiency of single cells was greatly reduced as compared with their plating efficiency in a medium containing dialyzed serum instead of whole serum, and the clones which did develop grew at a slower rate. The nutritional deficiency could be overcome by adding the seven amino acids which are ordinarily not nutritionally essential. In most of the experiments serine alone sufficed.
一种添加了透析血清的基本生长培养基，足以支持多种人类细胞系在高密度接种的单层培养和悬浮培养中生长，但不允许源自悬浮培养的少量海拉细胞、海拉S3细胞、结膜细胞或KB细胞正常或最佳生长。在血清阈值浓度下，与在含有透析血清而非全血清的培养基中相比，单细胞的接种效率大大降低，并且形成的克隆生长速度较慢。通过添加七种通常并非营养必需的氨基酸可以克服营养缺陷。在大多数实验中，仅丝氨酸就足够了。
