Opanasenko V K, Red'ko T P, Gubanova O N, Yaguzhinsky L S
Soil Science and Photosynthesis Institute, Russian Academy of Sciences, Pushchino, Moscow Region.
FEBS Lett. 1992 Aug 3;307(3):280-2. doi: 10.1016/0014-5793(92)80695-d.
The effect of N,N'-dicyclohexylcarbodiimide (DCCD) on photosynthetic electron transport and light-induced NH4+ and K+ uptake in the presence of ammonium or nigericin was studied. DCCD alone had no effect on either the electron transport or the uptake of protons. The simultaneous action of DCCD and low concentrations of ammonium or nigericin was shown to lead to a significant increase in the electron transport rate and a decrease in the steady-state uptake value of H+ and NH4+ or K+. The effect of DCCD on these processes was compared with the effect of the ionophore, valinomycin, which transports potassium and ammonium cations through membranes. The conclusion was made that 1.0-1.5 mol DCCD per mol chlorophyll activated the transfer system of monovalent cations (K+ and NH4+) in thylakoid membranes.
研究了N，N'-二环己基碳二亚胺（DCCD）在存在铵或尼日利亚菌素的情况下对光合电子传递以及光诱导的NH4+和K+吸收的影响。单独的DCCD对电子传递或质子吸收均无影响。结果表明，DCCD与低浓度的铵或尼日利亚菌素同时作用会导致电子传递速率显著增加，同时H+、NH4+或K+的稳态吸收值降低。将DCCD对这些过程的影响与离子载体缬氨霉素的影响进行了比较，缬氨霉素可使钾和铵阳离子通过膜运输。得出的结论是，每摩尔叶绿素1.0 - 1.5摩尔的DCCD可激活类囊体膜中单价阳离子（K+和NH4+）的转运系统。
