Zolotareva E K, Dovbysh E F, Tereshchenko A F, Onoiko E B
N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kiev.
FEBS Lett. 1997 Jul 28;412(2):375-8. doi: 10.1016/s0014-5793(97)00783-7.
It is shown that the inhibitory effect of N,N'-dicyclohexylcarbodiimide (DCCD) on photophosphorylation and uncoupled electron transfer from H2O to methylviologen (MV) in pea chloroplasts depends upon solvent concentration. Being applied as a solution in dimethyl sulfoxide (DMSO) DCCD did not suppress uncoupled electron transfer and inhibited photophosphorylation independently from DMSO concentration. If DCCD was applied as methanolic or ethanolic solution its concentration sufficient for half-maximum inhibition [I]50 of both photophosphorylation and uncoupled electron transfer decreased at increasing alcohol content. The data suggest that the synergistic effect of DCCD and alcohols is connected with DCCD-catalyzed etherification of some carboxylic groups which are important for chloroplast electron transfer.
结果表明，N，N'-二环己基碳二亚胺（DCCD）对豌豆叶绿体中光磷酸化以及从H2O到甲基紫精（MV）的解偶联电子转移的抑制作用取决于溶剂浓度。当以二甲基亚砜（DMSO）溶液形式应用时，DCCD不会抑制解偶联电子转移，并且与DMSO浓度无关地抑制光磷酸化。如果将DCCD以甲醇或乙醇溶液形式应用，随着醇含量的增加，其对光磷酸化和解偶联电子转移的半最大抑制浓度[I]50会降低。数据表明，DCCD与醇的协同作用与DCCD催化的某些对叶绿体电子转移很重要的羧基的醚化有关。
