Tsuzuki S, Kondo A, Fushiki T, Sugimoto E
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
FEBS Lett. 1992 Aug 3;307(3):386-8. doi: 10.1016/0014-5793(92)80719-w.
Monitor peptide (CCK-releasing peptide) mRNA increased on the administration of CCK in rat pancreas and the AR4-2J pancreatic cell line. Subcutaneous injection of CCK into rats at 8 h intervals increased the level of monitor peptide mRNA in the pancreas. Concomitant injection of CCK antagonist CR-1409 strongly decreased it. The monitor peptide mRNA was also increased by CCK in AR4-2J cells and was decreased by the antagonist. These findings suggest that the plasma CCK induced by prolonged intake of a high protein diet may be responsible for the adaptative increase in the monitor peptide as well as exocrine proteases in the pancreas.
在大鼠胰腺和AR4-2J胰腺细胞系中，给予胆囊收缩素（CCK）后，监测肽（CCK释放肽）mRNA水平升高。以8小时间隔给大鼠皮下注射CCK，可使胰腺中监测肽mRNA水平升高。同时注射CCK拮抗剂CR-1409可使其显著降低。在AR4-2J细胞中，CCK也可使监测肽mRNA水平升高，而拮抗剂可使其降低。这些发现表明，长期摄入高蛋白饮食诱导的血浆CCK可能是胰腺中监测肽以及外分泌蛋白酶适应性增加的原因。
