Rossitto P V, MacLachlan N J
Department of Veterinary Medical Pathology, University of California, Davis 95616.
J Gen Virol. 1992 Aug;73 ( Pt 8):1947-52. doi: 10.1099/0022-1317-73-8-1947.
Neutralizing epitopes present on the five serotypes of bluetongue virus (BTV) which have been isolated in the United States were investigated with a panel of monoclonal antibodies (MAbs). Neutralizing MAbs were raised against the U.S. prototype viruses of BTV serotypes 2, 10, 11, 13 and 17, and were reacted with each virus in both neutralization and immune precipitation assays. All MAbs neutralized and precipitated VP2 of the virus against which they were raised. Five MAbs raised against BTV-10 also precipitated VP2 of the prototype strain of BTV-17, and four of these MAbs neutralized BTV-17. To characterize further the neutralizing epitopes of BTV, the MAbs raised against BTV-10 and BTV-17 were reacted by immune precipitation and neutralization assays with four field strains each of BTV-17 and BTV-10 isolated from ruminants in the U.S. All MAbs raised against BTV-10 both precipitated VP2 and neutralized the four field isolates of BTV-10, whereas none of the MAbs raised against BTV-17 reacted with these viruses. By contrast, all seven MAbs raised against BTV-17 and four of the seven MAbs raised against BTV-10 precipitated VP2 of the four BTV-17 field isolates. Another MAb raised against BTV-10 precipitated VP2 of three of the four field isolates of BTV-17. Whereas neutralization of the BTV-17 field isolates by several MAbs was inconsistent, all 10 isolates of BTV-10 and BTV-17 were neutralized by three MAbs raised against BTV-10. Results of this and other studies indicate that multiple neutralizing epitopes exist on each serotype of BTV. Some of these epitopes are conserved whereas others apparently vary in their significance to the neutralization of individual field isolates of BTV-17 and perhaps other BTV serotypes. These findings have implications for the future development of efficacious subunit vaccines to prevent BTV infection of ruminants.
利用一组单克隆抗体（MAb）对在美国分离出的蓝舌病病毒（BTV）五种血清型上存在的中和表位进行了研究。针对BTV血清型2、10、11、13和17的美国原型病毒制备了中和单克隆抗体，并使其在中和试验和免疫沉淀试验中与每种病毒发生反应。所有单克隆抗体均能中和并沉淀其针对的病毒的VP2。针对BTV - 10制备的五种单克隆抗体也能沉淀BTV - 17原型株的VP2，其中四种单克隆抗体能中和BTV - 17。为了进一步表征BTV的中和表位，将针对BTV - 10和BTV - 17制备的单克隆抗体通过免疫沉淀试验和中和试验与从美国反刍动物中分离出的BTV - 17和BTV - 10的四个田间毒株进行反应。所有针对BTV - 10制备的单克隆抗体均能沉淀VP2并中和BTV - 10的四个田间分离株，而针对BTV - 17制备的单克隆抗体均未与这些病毒发生反应。相比之下，针对BTV - 17制备的所有七种单克隆抗体以及针对BTV - 10制备并能沉淀四种BTV - 17田间分离株VP2的七种单克隆抗体中的四种。另一种针对BTV - 10制备的单克隆抗体能沉淀四种BTV - 17田间分离株中的三种的VP2。虽然几种单克隆抗体对BTV - 17田间分离株的中和作用不一致，但针对BTV - 10制备的三种单克隆抗体能中和所有10株BTV - 10和BTV - 17分离株。这项研究和其他研究的结果表明，BTV的每种血清型上都存在多个中和表位。其中一些表位是保守的，而其他表位对BTV - 17的各个田间分离株以及可能对其他BTV血清型的中和作用的重要性显然有所不同。这些发现对未来开发预防反刍动物BTV感染的有效亚单位疫苗具有启示意义。
