蓝舌病病毒中和抗原的构象依赖性表位

Conformationally dependent epitopes of bluetongue virus neutralizing antigen.

作者信息

Grieder F B, Schultz K T

机构信息

Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.

出版信息

Viral Immunol. 1989;2(1):17-24. doi: 10.1089/vim.1989.2.17.

DOI:10.1089/vim.1989.2.17

PMID:2472808

Abstract

The neutralizing epitopes of Bluetongue virus serotype 17 (BTV 17) were evaluated by virus neutralization assay, flow cytometry, radioimmuno-precipitation, and Western blot. We showed that neutralizing monoclonal antibodies (MABs) raised to BTV 17 bound to viral proteins on intact virus when examined by virus neutralization and by an indirect binding assay analyzed by flow cytometry. In contrast, when the viral proteins were solubilized, a loss in reactivity with some of the neutralizing MABs was observed. Additionally, when the viral proteins were subjected to denaturing conditions, none of the neutralizing MABs reacted with the viral proteins. The results indicate that these neutralizing MABs bind conformationally dependent epitopes.

摘要

通过病毒中和试验、流式细胞术、放射免疫沉淀和蛋白质印迹法对蓝舌病毒17型（BTV 17）的中和表位进行了评估。我们发现，通过病毒中和试验以及流式细胞术分析的间接结合试验检测时，针对BTV 17产生的中和单克隆抗体（MABs）与完整病毒上的病毒蛋白结合。相比之下，当病毒蛋白溶解后，观察到一些中和MABs的反应性丧失。此外，当病毒蛋白处于变性条件下时，没有一种中和MABs与病毒蛋白发生反应。结果表明，这些中和MABs结合构象依赖性表位。