Lowenstein C J, Glatt C S, Bredt D S, Snyder S H
Department of Neuroscience, Johns Hopkins Medical Institutions, Baltimore, MD 21205.
Proc Natl Acad Sci U S A. 1992 Aug 1;89(15):6711-5. doi: 10.1073/pnas.89.15.6711.
Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.
一氧化氮（NO）是巨噬细胞、血管内皮细胞和神经元的信使分子。此前已克隆出一种神经元形式的一氧化氮合酶（NOS）。我们现在报告巨噬细胞NOS的分子克隆。巨噬细胞酶与神经元酶的序列同一性为50%。与神经元NOS一样，巨噬细胞NOS具有FAD、FMN和NADPH的识别位点，也具有一个共有钙调蛋白结合位点。巨噬细胞NOS mRNA具有显著的可诱导性；在静止的巨噬细胞或脾脏中不存在，但在内毒素处理后2 - 6小时显著表达。
