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兔骨骼肌丝裂原活化蛋白激酶（MAP激酶）激活剂的复性及部分肽段测序

Renaturation and partial peptide sequencing of mitogen-activated protein kinase (MAP kinase) activator from rabbit skeletal muscle.

作者信息

Wu J, Michel H, Rossomando A, Haystead T, Shabanowitz J, Hunt D F, Sturgill T W

机构信息

Department of Medicine, University of Virginia, Charlottesville 22908.

出版信息

Biochem J. 1992 Aug 1;285 ( Pt 3)(Pt 3):701-5. doi: 10.1042/bj2850701.

DOI:10.1042/bj2850701
PMID:1379797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132850/
Abstract

Mitogen-activated protein kinase (MAP kinase) activator was purified 2000-fold from skeletal muscle, and proteins which co-purified with the activator were analysed after SDS/PAGE by renaturation and partial sequencing. Activity for tyrosine and threonine phosphorylation of MAP kinase was present in two bands of approx. 48 and 46 kDa, which have sequence similarity to small GTP-binding protein p25 GDP dissociation inhibitor and protein kinases (PBS2, SPK1+, STE7, BYR1) respectively.

摘要

丝裂原活化蛋白激酶（MAP激酶）激活剂从骨骼肌中纯化了2000倍，并通过复性和部分测序对与该激活剂共纯化的蛋白质进行了SDS/PAGE分析。MAP激酶酪氨酸和苏氨酸磷酸化活性存在于两条约48 kDa和46 kDa的条带中，它们分别与小GTP结合蛋白p25 GDP解离抑制剂和蛋白激酶（PBS2、SPK1+、STE7、BYR1）具有序列相似性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec51/1132850/16743ab9fa1a/biochemj00130-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec51/1132850/f3c8a4729648/biochemj00130-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec51/1132850/16743ab9fa1a/biochemj00130-0033-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec51/1132850/f3c8a4729648/biochemj00130-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec51/1132850/16743ab9fa1a/biochemj00130-0033-b.jpg

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本文引用的文献

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Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.从十二烷基硫酸钠 - 聚丙烯酰胺凝胶中洗脱蛋白质、去除十二烷基硫酸钠以及酶活性的复性：大肠杆菌RNA聚合酶σ亚基、小麦胚芽DNA拓扑异构酶及其他酶的实验结果
Anal Biochem. 1980 Nov 15;109(1):76-86. doi: 10.1016/0003-2697(80)90013-5.
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Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.酵母调节基因STE7的核苷酸序列预测出一种与蛋白激酶同源的蛋白质。
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7371-5. doi: 10.1073/pnas.83.19.7371.
3
在非洲爪蟾卵母细胞中，p42丝裂原活化蛋白激酶（MAPK）的激活而非c-Jun氨基末端激酶的激活，可诱导MAPK磷酸酶XCL100的磷酸化和稳定化。
Mol Biol Cell. 2002 Feb;13(2):454-68. doi: 10.1091/mbc.01-11-0553.
4
Distinct, constitutively active MAPK phosphatases function in Xenopus oocytes: implications for p42 MAPK regulation In vivo.不同的组成型活性丝裂原活化蛋白激酶磷酸酶在非洲爪蟾卵母细胞中发挥作用：对体内p42丝裂原活化蛋白激酶调控的影响。
Mol Biol Cell. 1999 Nov;10(11):3729-43. doi: 10.1091/mbc.10.11.3729.
5
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Protein sequencing by tandem mass spectrometry.
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J Biol Chem. 1990 Feb 5;265(4):2333-7.