Mora M L, Santiago E, Montesinos J J, Weiss-Steider B
Laboratory of Cellular Differentiation and Cancer, National University of Mexico.
Eur Cytokine Netw. 1992 May-Jun;3(3):337-41.
The induction of granulocyte and macrophage colony formation by the granulocyte-macrophage colony stimulating factor (GM-CSF) on bone marrow cells (BMC) was evaluated as a function of time in agar cultures. We found that while macrophage cell clusters were very abundant on the first two days of culture, granulocytic cell clusters did not appear until the third day. We also found that macrophage colonies were present from the fourth day of culture, while granulocyte colonies did not appear until the fifth day. When two day cell clusters were transferred to cultures with GM-CSF we observed that only macrophage-colonies developed. On the other hand, when four day clusters were transferred, both granulocyte and macrophage colony formation was obtained in a similar way as the one obtained when using GM-CSF with fresh BMC. Two day clusters did not respond to granulocyte colony stimulating factor (G-CSF) while fourth day clusters generated granulocytic colonies in a similar way as when G-CSF was used with fresh BMC. In order to test the hypothesis that granulocyte colony formation in these assays could be a result of the secretion of G-CSF by the macrophages previously induced by GM-CSF, lysates from macrophage colonies were used to induce colony formation on BMC. We observed that colonies, mainly granulocytic, were induced in a similar way as when G-CSF was used. Finally, the possibility that GM-CSF is just a macrophage inducer with the property to produce cells that secrete G-CSF is discussed.
在琼脂培养中，评估粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）对骨髓细胞（BMC）诱导粒细胞和巨噬细胞集落形成的时间函数。我们发现，虽然在培养的前两天巨噬细胞簇非常丰富，但粒细胞簇直到第三天才出现。我们还发现，巨噬细胞集落从培养的第四天开始出现，而粒细胞集落直到第五天才出现。当将两天的细胞簇转移到含有GM - CSF的培养物中时，我们观察到只有巨噬细胞集落形成。另一方面，当转移四天的细胞簇时，粒细胞和巨噬细胞集落形成的方式与使用GM - CSF和新鲜BMC时相似。两天的细胞簇对粒细胞集落刺激因子（G - CSF）无反应，而四天的细胞簇产生粒细胞集落的方式与使用G - CSF和新鲜BMC时相似。为了检验在这些试验中粒细胞集落形成可能是由GM - CSF预先诱导的巨噬细胞分泌G - CSF所致的假设，使用巨噬细胞集落的裂解物诱导BMC上的集落形成。我们观察到，主要是粒细胞集落，其诱导方式与使用G - CSF时相似。最后，讨论了GM - CSF是否只是一种巨噬细胞诱导剂，具有产生分泌G - CSF细胞的特性。
