Laboratorio de Hematopoyesis y Leucemia, UNAM, Mexico, D.F., Mexico.
Inflamm Res. 2012 Apr;61(4):367-73. doi: 10.1007/s00011-011-0421-7. Epub 2012 Jan 20.
Sodium caseinate (CasNa) induces differentiation and M-CSF production in mouse band granulocytes in vitro; however, it is not yet known if this molecule can also induce the proliferation and activation of the granulocyte lineage in vivo. In this work we evaluated the induction in vivo of granulopoiesis and the activation of granulocytes in mice treated with CasNa.
BALB/c male mice 8-12 weeks old were used.
The animals were inoculated intraperitoneally with 1 ml of CasNa (10% in PBS p/v) four times (every 48 h).
Granulocyte proliferation was evaluated by flow cytometry; activation was evaluated by phagocytic indices. The cytokine was measured using an ELISA assay.
We show that CasNa increased bone marrow granulopoiesis percentage (38.35 ± 10.88 vs. 64.94 ± 34.14 BrdU+/Gr-1+ cells) and the granulocytes generated presented increased phagocytic indices (0.3 ± 0.1 vs. 0.6 ± 0.11, p < 0.05). We also show that G-CSF (974 ± 411 vs. 3189 ± 350 pg/ml, p < 0.05) and GM-CSF increased in serum, but only G-CSF in bone marrow plasma.
CasNa induces granulopoiesis with functional granulocytes, suggesting that this molecule could be an innate immune system activator.
酪蛋白酸钠（CasNa）在体外诱导小鼠带颗粒白细胞分化和产生 M-CSF；然而，目前尚不清楚该分子是否也能在体内诱导粒细胞系的增殖和激活。在这项工作中，我们评估了 CasNa 处理的小鼠体内粒细胞生成的诱导和粒细胞的激活。
使用 8-12 周龄 BALB/c 雄性小鼠。
动物经腹腔内接种 1ml CasNa（10%在 PBS p/v）四次（每 48 小时一次）。
通过流式细胞术评估粒细胞增殖；通过吞噬指数评估激活。使用 ELISA 测定法测量细胞因子。
我们表明 CasNa 增加了骨髓粒细胞生成百分比（38.35±10.88 对 64.94±34.14 BrdU+/Gr-1+细胞），产生的粒细胞表现出增加的吞噬指数（0.3±0.1 对 0.6±0.11，p<0.05）。我们还表明，血清中 G-CSF（974±411 对 3189±350 pg/ml，p<0.05）和 GM-CSF 增加，但仅骨髓血浆中 G-CSF 增加。
CasNa 诱导具有功能粒细胞的粒细胞生成，表明该分子可能是先天免疫系统的激活剂。
