Karaseva E I, Eremin A N, Metelitsa D I
Bioorg Khim. 1992 Apr;18(4):498-508.
Conjugates containing 1, 6 or 11 cortisol molecules per peroxidase molecule were obtained by the reaction of the N-hydroxysuccinimide ester of cortisol 3-O-carboxymethyloxime (COR) with horse-radish peroxidase (HRP). Activities of the peroxidase conjugates and their immunocomplexes with antibodies against cortisol in the orthophenylenediamine oxidation in reversed Aerosol OT micelles in heptane at various hydration degrees of the micelles (omega 0) were studied. Catalytic activities of HRP and its conjugates, on one hand, and of the immunocomplexes of HRP with anti-HRP and the conjugates with antibodies against cortisol, on the other hand, differed significantly and depended in a different manner on the hydration degree of AOT micelles. These differences became the basis of a homogeneous enzyme-immunoassay of cortisol in the reversed micelles of AOT in heptane.
通过皮质醇3 - O - 羧甲基肟（COR）的N - 羟基琥珀酰亚胺酯与辣根过氧化物酶（HRP）反应，获得了每个过氧化物酶分子含有1、6或11个皮质醇分子的共轭物。研究了在庚烷中反相气溶胶OT胶束在不同水合度（ω0）下，过氧化物酶共轭物及其与抗皮质醇抗体的免疫复合物在邻苯二胺氧化反应中的活性。一方面，HRP及其共轭物的催化活性，另一方面，HRP与抗HRP的免疫复合物以及共轭物与抗皮质醇抗体的免疫复合物的催化活性存在显著差异，并且以不同方式依赖于AOT胶束的水合度。这些差异成为了在庚烷中AOT反相胶束中进行皮质醇均相酶免疫测定的基础。
