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双（2-乙基己基）磺基琥珀酸钠（AOT）在水溶液中以及在AOT/正庚烷反胶束中与过氧化氢酶和辣根过氧化物酶的相互作用。

Interaction of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) with catalase and horseradish peroxidase in an aqueous solution and in the reverse micelles of AOT/N-heptane.

作者信息

Gebicka L, Gebicki J L

机构信息

Institute of Applied Radiation Chemistry, Technical University of Lódź, Poland.

出版信息

Biochem Mol Biol Int. 1998 Jul;45(4):805-11.

PMID:9713705

Abstract

Some properties of catalase and horseradish peroxidase entrapped into reverse micelles of sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/n-heptane with those in an aqueous solution of AOT are compared. Secondary structure of catalase significantly changes and activity is completely lost in the presence of aqueous micellar solution of AOT. In AOT/n-heptane reverse micelles secondary structure of catalase does not change. AOT has no effect on horseradish peroxidase in aqueous solution. On the other hand slight changes in secondary structure of horseradish peroxidase in AOT/n-heptane reverse micelles appear. It is concluded that electrostatic interactions between catalase or horseradish peroxidase with AOT molecule at neutral pH are not the main factor determining reactivity of investigated enzymes in reverse micelles.

摘要

比较了 entrapped 于双（2-乙基己基）磺基琥珀酸钠（AOT）/正庚烷反胶束中的过氧化氢酶和辣根过氧化物酶的一些性质与它们在 AOT 水溶液中的性质。在 AOT 胶束水溶液存在的情况下，过氧化氢酶的二级结构发生显著变化且活性完全丧失。在 AOT/正庚烷反胶束中，过氧化氢酶的二级结构没有变化。AOT 对水溶液中的辣根过氧化物酶没有影响。另一方面，在 AOT/正庚烷反胶束中辣根过氧化物酶的二级结构出现轻微变化。得出的结论是，在中性 pH 下，过氧化氢酶或辣根过氧化物酶与 AOT 分子之间的静电相互作用不是决定所研究酶在反胶束中反应活性的主要因素。