Wilson A C, Murtadha M, Wakil S J
Atherosclerosis. 1977 Jan;26(1):103-15. doi: 10.1016/0021-9150(77)90145-9.
Fatty acid synthesis by subcellular fractions of human aorta was studied by measuring the incorporation of either radioactive acetyl-CoA or malonyl-CoA into long chain fatty acids. The high speed supernatant fraction contained fatty acid synthetase and was capable of de novo fatty acid synthesis. The fatty acid synthetase from chicken aorta was purified 800-fold from the high speed supernatant and was judged to be 10% pure at this level. Its molecular weight was estimated to be 450,000 on the basis of agarose gel filtration chromatography, while under dissociating conditions a molecular weight of 220,000 was obtained on sodium dodecyl sulphate disc gel electrophoresis. Fatty acid synthesis was dependent on acetyl-CoA, malonyl-CoA and NADPH. The major product was free palmitic acid. In enzymatic and physical characteristics the chicken aorta fatty acid synthetase strongly resembles the synthetase isolated from chicken liver. The two enzymes cross-react immuno-chemically and this homology provides the possibility of studying the synthesis and degradation of the aorta synthetase during the development of atherosclerosis.
通过测量放射性乙酰辅酶A或丙二酰辅酶A掺入长链脂肪酸的量，研究了人主动脉亚细胞组分的脂肪酸合成。高速上清液组分含有脂肪酸合成酶，能够从头合成脂肪酸。从鸡主动脉中提取的脂肪酸合成酶从高速上清液中纯化了800倍，此时纯度被判定为10%。根据琼脂糖凝胶过滤色谱法估计其分子量为450,000，而在解离条件下，十二烷基硫酸钠圆盘凝胶电泳得到的分子量为220,000。脂肪酸合成依赖于乙酰辅酶A、丙二酰辅酶A和NADPH。主要产物是游离棕榈酸。在酶学和物理特性方面，鸡主动脉脂肪酸合成酶与从鸡肝脏中分离的合成酶非常相似。这两种酶在免疫化学上发生交叉反应，这种同源性为研究动脉粥样硬化发展过程中主动脉合成酶的合成和降解提供了可能性。
