Seyama Y, Otsuka H, Kawaguchi A, Yamakawa T
J Biochem. 1981 Sep;90(3):789-97. doi: 10.1093/oxfordjournals.jbchem.a133535.
Fatty acid synthetase was isolated from guinea pig Harderian gland. This enzyme complex exhibited a unique character as compared with the fatty acid synthetase from the liver of the same animal. The former enzyme produced many odd-numbered and methyl-branched fatty acids in the presence of methylmalonyl-CoA. These fatty acids are characteristic components of the lipid secreted from this gland. The chemical structure of this lipid has been identified as 1-O-alkyl-2,3-diacylglycerol by previous work from this laboratory (Yamazaki, T., Seyama, Y., Otsuka, H., Ogawa, H., & Yamakawa, T. (1981) J. Biochem. 89, 683-691). Apparent Km values (5 X 10(-6) M) for acetyl-CoA and propionyl-CoA were the same, but the Vmax for propionyl-CoA was much higher than that for acetyl-CoA. The pI value of the fatty acid synthetase from Harderian gland was 5.3, and the molecular weight of the enzyme was 9 X 10(5) daltons. The beta-ketoacyl reductase had pro-S stereospecificity and the enoly reductase had pro-R stereospecificity for NADPH. The results presented in this paper indicate that the fatty acid synthetase from guinea pig Harderian gland can produce a set of fatty acids needed for the synthesis of the lipid secreted from this gland, and that the fatty acid synthetase has a characteristic organ specificity.
脂肪酸合成酶是从豚鼠哈德氏腺中分离出来的。与同一动物肝脏中的脂肪酸合成酶相比，这种酶复合物表现出独特的特性。前一种酶在甲基丙二酰辅酶A存在的情况下会产生许多奇数碳和甲基支链脂肪酸。这些脂肪酸是该腺体分泌的脂质的特征性成分。根据本实验室之前的研究工作（山崎，T.，关山，Y.，大冢，H.，小川，H.，&山川，T.（1981年）《生物化学杂志》89卷，683 - 691页），这种脂质的化学结构已被确定为1 - O - 烷基 - 2,3 - 二酰基甘油。乙酰辅酶A和丙酰辅酶A的表观Km值（5×10⁻⁶ M）相同，但丙酰辅酶A的Vmax远高于乙酰辅酶A。哈德氏腺脂肪酸合成酶的pI值为5.3，该酶的分子量为9×10⁵道尔顿。β - 酮酰还原酶对NADPH具有前 - S立体特异性，烯醇还原酶对NADPH具有前 - R立体特异性。本文给出的结果表明，豚鼠哈德氏腺的脂肪酸合成酶能够产生该腺体分泌的脂质合成所需的一组脂肪酸，并且该脂肪酸合成酶具有特征性的器官特异性。
