• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Bleomycin mediated degradation of DNA-RNA hybrids does not involve C-1' chemistry.博来霉素介导的DNA-RNA杂交体降解不涉及C-1'化学过程。
Nucleic Acids Res. 1992 Aug 25;20(16):4179-85. doi: 10.1093/nar/20.16.4179.
2
Mechanism of bleomycin: evidence for a rate-determining 4'-hydrogen abstraction from poly(dA-dU) associated with the formation of both free base and base propenal.
Biochemistry. 1985 Dec 17;24(26):7562-8. doi: 10.1021/bi00347a009.
3
The mechanism of free base formation from DNA by bleomycin. A proposal based on site specific tritium release from Poly(dA.dU).博来霉素作用于DNA形成游离碱的机制。基于从聚（dA.dU）中位点特异性释放氚的一项提议。
J Biol Chem. 1983 Apr 25;258(8):4694-7.
4
Mechanism of bleomycin: evidence for 4'-ketone formation in poly(dA-dU) associated exclusively with free base release.
Biochemistry. 1985 Dec 17;24(26):7569-73. doi: 10.1021/bi00347a010.
5
DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.
Biochemistry. 1986 Oct 21;25(21):6586-92. doi: 10.1021/bi00369a037.
6
Determination of the kinetics of deuteration of DNA.RNA hybrids by ultraviolet spectroscopy.通过紫外光谱法测定DNA-RNA杂交体的氘代动力学。
Biochemistry. 1985 Aug 27;24(18):4823-5. doi: 10.1021/bi00339a016.
7
Reaction of DNA-bound ferrous bleomycin with dioxygen: activation versus stabilization of dioxygen.
Biochemistry. 1994 May 3;33(17):5319-27. doi: 10.1021/bi00183a040.
8
Comparison of base inclination of ribo-GC and deoxyribo-GC polymers, and synthesis of poly(rGrC)-poly(rGrC).核糖核酸 - 鸟嘌呤 - 胞嘧啶（ribo - GC）和脱氧核糖核酸 - 鸟嘌呤 - 胞嘧啶（deoxyribo - GC）聚合物的碱基倾斜度比较以及聚（rGrC）-聚（rGrC）的合成。
Biopolymers. 1995 Sep;36(3):313-22. doi: 10.1002/bip.360360306.
9
Monte-Carlo simulation of DNA duplex hydration. B and B' conformations of poly(dA).poly(dT) have different hydration shells.DNA双链水合作用的蒙特卡罗模拟。聚（dA）·聚（dT）的B和B'构象具有不同的水合壳层。
J Biomol Struct Dyn. 1988 Dec;6(3):575-86. doi: 10.1080/07391102.1988.10506508.
10
Dependence of the hydration shell structure in the minor groove of the DNA double helix on the groove width as revealed by Monte Carlo simulation.通过蒙特卡罗模拟揭示DNA双螺旋小沟中水合壳层结构对沟宽的依赖性。
Biopolymers. 1991 Oct 15;31(12):1445-53. doi: 10.1002/bip.360311211.

引用本文的文献

1
Effects of hypoxanthine substitution on bleomycin-mediated DNA strand degradation in DNA-RNA hybrids.次黄嘌呤替代对博来霉素介导的DNA-RNA杂交体中DNA链降解的影响。
Nucleic Acids Res. 1997 May 1;25(9):1846-53. doi: 10.1093/nar/25.9.1846.
2
Mechanistic analyses of site-specific degradation in DNA-RNA hybrids by prototypic DNA cleavers.原型DNA切割剂对DNA-RNA杂交体中位点特异性降解的机制分析。
Nucleic Acids Res. 1997 May 1;25(9):1836-45. doi: 10.1093/nar/25.9.1836.

本文引用的文献

1
CONVERSION OF MONO- AND OLIGODEOXYRIBONUCLEOTIDES TO 5-TRIPHOSPHATES.单脱氧核糖核苷酸和寡脱氧核糖核苷酸向5-三磷酸酯的转化。
J Am Chem Soc. 1965 Apr 20;87:1785-8. doi: 10.1021/ja01086a031.
2
Synthesis and biological activity of a new class of cytotoxic agents: N-(3-oxoprop-1-enyl)-substituted pyrimidines and purines.
J Med Chem. 1984 Aug;27(8):954-8. doi: 10.1021/jm00374a004.
3
Identification of oligonucleotide fragments produced in a strand scission reaction of the d(C-G-C-G-C-G) duplex by bleomycin.博来霉素引发的d（C-G-C-G-C-G）双链体链断裂反应中产生的寡核苷酸片段的鉴定。
Nucleic Acids Res. 1984 Feb 10;12(3):1581-92. doi: 10.1093/nar/12.3.1581.
4
The mechanism of free base formation from DNA by bleomycin. A proposal based on site specific tritium release from Poly(dA.dU).博来霉素作用于DNA形成游离碱的机制。基于从聚（dA.dU）中位点特异性释放氚的一项提议。
J Biol Chem. 1983 Apr 25;258(8):4694-7.
5
Polynucleotide helix geometry and stability. Spectroscopic, antigenic and interferon-inducing properties of deoxyribose-, ribose-, or 2'-deoxy-2'-fluororibose-containing duplexes of poly(inosinic acid) . poly(cytidylic acid).多核苷酸螺旋结构与稳定性。聚（肌苷酸）·聚（胞苷酸）中含脱氧核糖、核糖或2'-脱氧-2'-氟核糖的双链体的光谱、抗原及诱导干扰素特性 。
J Biol Chem. 1982 Feb 25;257(4):1924-8.
6
Bleomycin-induced strand-scission of DNA. Mechanism of deoxyribose cleavage.博来霉素诱导的DNA链断裂。脱氧核糖裂解机制。
J Biol Chem. 1981 Aug 25;256(16):8608-15.
7
Origin of malondialdehyde from DNA degraded by Fe(II) x bleomycin.铁（II）博来霉素降解DNA产生丙二醛的来源。
J Biol Chem. 1980 Dec 25;255(24):11832-8.
8
A novel method for phosphorylation of nucleosides to 5'-nucleotides.一种将核苷磷酸化为5'-核苷酸的新方法。
Tetrahedron Lett. 1967 Dec;50:5065-8. doi: 10.1016/s0040-4039(01)89915-9.
9
Thin-layer chromatography of aldonic acid lactones, aldoses and alditols.
J Chromatogr. 1967 Jan;26(1):116-20. doi: 10.1016/s0021-9673(01)98844-2.
10
Molecular and crystal structure of the polynucleotide complex: polyinosinic acid plus polydeoxycytidylic acid.多核苷酸复合物的分子结构与晶体结构：聚肌苷酸加聚脱氧胞苷酸
J Mol Biol. 1970 Mar 14;48(2):243-61. doi: 10.1016/0022-2836(70)90159-2.

博来霉素介导的DNA-RNA杂交体降解不涉及C-1'化学过程。

Bleomycin mediated degradation of DNA-RNA hybrids does not involve C-1' chemistry.

作者信息

Absalon M J, Krishnamoorthy C R, McGall G, Kozarich J W, Stubbe J

机构信息

Department of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Nucleic Acids Res. 1992 Aug 25;20(16):4179-85. doi: 10.1093/nar/20.16.4179.

DOI:10.1093/nar/20.16.4179
PMID:1380696
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334123/
Abstract

Incubation of Fe(II) bleomycin and O2 with a number of 'A'-like DNA-RNA hybrid homopolymers at 4 atm O2 results in formation of base propenal and base in a ratio of approximately 1.0:1.0. This ratio differs dramatically from the corresponding ratio of approximately 10:1.0 observed when activated BLM degrades 'B'-like DNA homopolymers. Experiments were undertaken to determine if the shift to enhanced base production observed in the A-like hybrids is the result of C-1' chemistry in addition to the C-4' chemistry normally observed with B-like DNA under identical conditions. Increased accessibility of the 1'-hydrogen might be anticipated due to widening of the minor groove in the A-like conformers. Experiments using poly([1'-3H]dA) poly(rU) and poly([U-14C]dA) poly(rU) indicated that neither 3H2O nor deoxyribonolactone accompanied adenine release. In addition, studies using poly([4'-2H]dA) poly(rU) and poly([1'-2H]dA) poly(rU) unambiguously establish that the altered base to base propenal ratio is not the result of C-1' chemistry, but a direct consequence of C-4' chemistry.

摘要

在4个大气压的氧气条件下，将亚铁博来霉素和氧气与多种“A”型DNA - RNA杂交同聚物一起温育，会导致碱基丙烯醛和碱基以大约1.0:1.0的比例形成。该比例与活化的博来霉素降解“B”型DNA同聚物时观察到的大约10:1.0的相应比例有显著差异。进行实验以确定在“A”型杂交物中观察到的碱基产量增加是否除了在相同条件下通常在“B”型DNA中观察到的C - 4'化学作用外，还是C - 1'化学作用的结果。由于“A”型构象中窄小沟的变宽，可能预期1'-氢的可及性增加。使用聚（[1'-³H]dA）聚（rU）和聚（[U - ¹⁴C]dA）聚（rU）的实验表明，腺嘌呤释放时既没有³H₂O也没有脱氧核糖内酯伴随。此外，使用聚（[4'-²H]dA）聚（rU）和聚（[1'-²H]dA）聚（rU）的研究明确证实，碱基与碱基丙烯醛比例的改变不是C - 1'化学作用的结果，而是C - 4'化学作用的直接后果。