Uesugi S, Shida T, Ikehara M, Kobayashi Y, Kyogoku Y
Nucleic Acids Res. 1984 Feb 10;12(3):1581-92. doi: 10.1093/nar/12.3.1581.
To elucidate the mechanism of DNA strand scission by bleomycin, a d(C-G-C-G-C-G) duplex was treated with the bleomycin-iron ion complex in the presence of H2O2 and degradation products (1, 2, cytosine and deoxyguanosine 5'-phosphate) were identified. 1 and 2 contain a carboxymethyl group attached to the 3'-terminal phosphoryl group of d(C-Gp) and d(C-G-C-Gp), respectively. These compounds were identified by UV, 1H and 31P NMR spectroscopy and paper electrophoresis. 1 was synthesized from the protected dinucleotide and glycolic acid and the proton NMR spectrum was identical to that of 1 obtained as a degradation product. Thus the oligonucleotide fragments produced by the action of bleomycin on DNA were directly identified and cleavage of the C3'-C4' bond of the sugar residues was proved.
为阐明博来霉素导致DNA链断裂的机制，在过氧化氢存在的情况下，用博来霉素 - 铁离子复合物处理d（C-G-C-G-C-G）双链体，并鉴定降解产物（1、2、胞嘧啶和脱氧鸟苷5'-磷酸）。1和2分别含有连接到d（C-Gp）和d（C-G-C-Gp）的3'-末端磷酰基上的羧甲基。通过紫外光谱、1H和31P核磁共振光谱以及纸电泳对这些化合物进行了鉴定。1由受保护的二核苷酸和乙醇酸合成，其质子核磁共振光谱与作为降解产物获得的1的光谱相同。因此，直接鉴定了博来霉素作用于DNA产生的寡核苷酸片段，并证明了糖残基的C3'-C4'键发生了断裂。
