Higashimoto Y, Simchock J, Liddle R A
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Biochim Biophys Acta. 1992 Aug 17;1132(1):72-4. doi: 10.1016/0167-4781(92)90054-4.
A cDNA clone encoding glucose-dependent insulinotropic peptide (GIP) was identified that consisted of 34 bp of 5' untranslated sequence, an open reading frame of 432 bp and 115 bp in the 3' untranslated region. The deduced amino acid sequence revealed a 144 amino acid preprohormone consisting of a 43 amino acid N-terminal extension including a signal peptide, a 42 amino acid hormone, and a 59 amino acid C-terminal extension. Rat GIP differs from the human hormone by two amino acid substitutions: arginine for histidine at position 18 and leucine for isoleucine at position 40. A single mRNA from small intestine of approximately 800 bases was identified on Northern blot analysis in equivalent amounts in proximal and distal small intestine.
鉴定出一个编码葡萄糖依赖性促胰岛素多肽（GIP）的cDNA克隆，它由34bp的5'非翻译序列、一个432bp的开放阅读框和3'非翻译区的115bp组成。推导的氨基酸序列显示为一个144个氨基酸的前激素原，由一个包含信号肽的43个氨基酸的N端延伸区、一个42个氨基酸的激素和一个59个氨基酸的C端延伸区组成。大鼠GIP与人激素在两个氨基酸位置上存在差异：第18位的组氨酸被精氨酸取代，第40位的异亮氨酸被亮氨酸取代。在Northern印迹分析中，从小肠中鉴定出一条约800个碱基的单一mRNA，在近端和远端小肠中的含量相当。
