从肿瘤细胞系（STC6 - 14）中分离小鼠葡萄糖依赖性促胰岛素多肽（GIP）cDNA，并对葡萄糖诱导的GIP mRNA增加进行定量分析。

Isolation of a murine glucose-dependent insulinotropic polypeptide (GIP) cDNA from a tumor cell line (STC6-14) and quantification of glucose-induced increases in GIP mRNA.

作者信息

Schieldrop P J, Gelling R W, Elliot R, Hewitt J, Kieffer T J, McIntosh C H, Pederson R A

机构信息

Department of Physiology, University of British Columbia, Vancouver, Canada.

出版信息

Biochim Biophys Acta. 1996 Aug 14;1308(2):111-3. doi: 10.1016/0167-4781(96)00103-0.

DOI:10.1016/0167-4781(96)00103-0

PMID:8764827

Abstract

A 537 base pair cDNA clone for murine GIP has been isolated. The elucidated sequence encodes an open reading frame of 432 base pairs which codes for a 144 amino acid precursor. Murine GIP is predicted to differ from the human hormone by three amino acid substitutions: arginine for histidine at position 18, arginine for lysine at position 30 and serine for lysine at position 34. GIP mRNA levels in STC6-14 cells incubated in the presence of varying glucose concentrations was investigated using a competitive-PCR method. In the presence of a 5-mM glucose stimulus, 1 x 10(5) GIP cells were found to contain 3.9 +/- 0.59 amol of GIP mRNA while the same number of cells contained 11.6 +/- 1.4 amol when subjected to a high (25 mM) glucose stimulus.

摘要

已分离出小鼠GIP的一个537个碱基对的cDNA克隆。所阐明的序列编码一个432个碱基对的开放阅读框，该开放阅读框编码一个144个氨基酸的前体。预测小鼠GIP与人激素在三个氨基酸取代上存在差异：第18位的组氨酸被精氨酸取代、第30位的赖氨酸被精氨酸取代以及第34位的赖氨酸被丝氨酸取代。使用竞争性PCR方法研究了在不同葡萄糖浓度下培养的STC6 - 14细胞中GIP mRNA的水平。在5 mM葡萄糖刺激下，发现1×10⁵个GIP细胞含有3.9±0.59 amol的GIP mRNA，而在高（25 mM）葡萄糖刺激下，相同数量的细胞含有11.6±1.4 amol。