Corless C L, Mendoza A, Collins T, Lawler J
Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115.
Dev Dyn. 1992 Apr;193(4):346-58. doi: 10.1002/aja.1001930408.
Thrombospondin is an adhesive glycoprotein that is thought to play a role in tissue genesis and repair. We have used a monoclonal anti-thrombospondin antibody, designated 5G11, to localize thrombospondin in paraformaldehyde fixed, paraffin-embedded sections of developing mouse embryos. Thrombospondin expression is observed in uterine smooth muscle, endometrial glands, the decidua, and trophoblastic giant cells during the initial phase of post-implantation development in the embryo. Cardiac myocytes and neuroepithelial cells show positive staining for thrombospondin at day 8.5 of gestation, and this expression continues throughout the development of the myocardium and central nervous system. Strong staining for thrombospondin is seen in developing bone and in the liver. Thrombospondin is also observed in developing smooth muscle and skeletal muscle, as well as in a variety of epithelia, including the epidermis, small intestinal epithelium, lens epithelium, renal tubular epithelium, and the epithelium of the developing tooth. Comparison of thrombospondin staining with that of two known cell surface receptors for thrombospondin, syndecan and the vitronectin receptor, reveals remarkable colocalization of thrombospondin and syndecan in all tissues, but almost no coexpression with the vitronectin receptor. Coexpression of thrombospondin and syndecan may play an important role in cell-cell or cell-matrix interactions during development.
血小板反应蛋白是一种黏附性糖蛋白，被认为在组织发生和修复中发挥作用。我们使用了一种名为5G11的抗血小板反应蛋白单克隆抗体，来定位发育中小鼠胚胎经多聚甲醛固定、石蜡包埋切片中的血小板反应蛋白。在胚胎植入后发育的初始阶段，在子宫平滑肌、子宫内膜腺、蜕膜和滋养层巨细胞中观察到血小板反应蛋白的表达。在妊娠第8.5天时，心肌细胞和神经上皮细胞对血小板反应蛋白呈阳性染色，并且这种表达在心肌和中枢神经系统的整个发育过程中持续存在。在发育中的骨骼和肝脏中可见血小板反应蛋白的强染色。在发育中的平滑肌和骨骼肌以及包括表皮、小肠上皮、晶状体上皮、肾小管上皮和发育中牙齿的上皮在内的各种上皮中也观察到血小板反应蛋白。血小板反应蛋白染色与两种已知的血小板反应蛋白细胞表面受体（多配体蛋白聚糖和玻连蛋白受体）的染色比较显示，在所有组织中血小板反应蛋白和多配体蛋白聚糖有明显的共定位，但几乎不与玻连蛋白受体共表达。血小板反应蛋白和多配体蛋白聚糖的共表达可能在发育过程中的细胞 - 细胞或细胞 - 基质相互作用中起重要作用。
