Hantaï D, Rao J S, Reddy B R, Festoff B W
Développement, Pathologie, Régénérationk du Système Neuromusculaire, INSERM U. 153, Paris, France.
Eur J Cell Biol. 1991 Aug;55(2):286-94.
Immunocytochemical localization of the adhesive glycoprotein thrombospondin made in comparison with other components of extracellular matrices shows its sequential appearance in the mouse muscle endomysium during postnatal development. Thrombospondin, absent at birth, in contrast to laminin, type IV collagen and fibronectin, is progressively detected during the first month of neonatal life in the whole muscle extracellular matrix. Immunoblotting of thrombospondin showed the appearance 14 days after birth of a band migrating at 180 kDa corresponding to thrombospondin. A fragment of thrombospondin at 110 kDa was already present at birth, as was also a lower molecular mass band at 70 kDa. Another band at 50 kDa also appeared during development in muscle extracts. Clonal muscle cells in culture were able to synthesize thrombospondin but only at the myotube stage, since little thrombospondin was detected at the myoblast stage. These data show a development regulation of thrombospondin expression in muscle which correlates with muscle differentiation.
通过与细胞外基质的其他成分进行比较，对黏附糖蛋白血小板反应蛋白进行免疫细胞化学定位，结果显示在出生后发育过程中，血小板反应蛋白在小鼠肌肉内膜中呈顺序性出现。与层粘连蛋白、IV型胶原和纤连蛋白不同，出生时不存在血小板反应蛋白，在新生生命的第一个月期间，在整个肌肉细胞外基质中逐渐检测到该蛋白。血小板反应蛋白的免疫印迹显示，出生后14天出现一条迁移率为180 kDa的条带，对应于血小板反应蛋白。出生时就已存在110 kDa的血小板反应蛋白片段，还有一条70 kDa的低分子量条带也是如此。在肌肉提取物的发育过程中还出现了一条50 kDa的条带。培养中的克隆肌肉细胞能够合成血小板反应蛋白，但仅在肌管阶段，因为在成肌细胞阶段几乎检测不到血小板反应蛋白。这些数据表明肌肉中血小板反应蛋白表达的发育调控与肌肉分化相关。
