Mimura K, Sueishi K, Yasunaga C, Tanaka K
Department of Pathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Invasion Metastasis. 1992;12(1):24-34.
We studied the role of the fibrinolytic function in the invasiveness of murine melanoma B16F1 and F10 cells using a reconstituted matrix on a filter in a modified Boyden chamber. The main species of plasminogen activators (PAs) synthesized in cell lysates and released into conditioned media by these cells was found to be tissue-type PA (t-PA). The invasiveness of these cell lines was enhanced by adding plasminogen to the gel matrix. This enhancing effect of plasminogen was markedly suppressed by adding anti-t-PA IgG and plasmin inhibitors into the gel matrix, but less affected by anti-urokinase-type PA (u-PA) IgG, offering more evidence to the hypothesis that the activation of the fibrinolytic system by PAs plays an important role in the invasiveness of murine melanoma B16 cell lines, and indicating that t-PA contributed more than u-PA to the invasive potential of these cells into the pericellular matrix.
我们使用改良的博伊登小室中滤膜上的重组基质，研究了纤溶功能在小鼠黑色素瘤B16F1和F10细胞侵袭性中的作用。发现这些细胞在细胞裂解物中合成并释放到条件培养基中的纤溶酶原激活剂（PA）的主要种类是组织型PA（t-PA）。通过向凝胶基质中添加纤溶酶原，这些细胞系的侵袭性增强。通过向凝胶基质中添加抗t-PA IgG和纤溶酶抑制剂，纤溶酶原的这种增强作用被显著抑制，但受抗尿激酶型PA（u-PA）IgG的影响较小，这为PA激活纤溶系统在小鼠黑色素瘤B16细胞系侵袭性中起重要作用的假说提供了更多证据，并表明t-PA对这些细胞向细胞周基质的侵袭潜力的贡献大于u-PA。
