Baramova E N, Coucke P, Leprince P, De Pauw-Gillet M C, Bassleer R, Foidart J M
University of Liège, Sart-Tilman, Belgium.
Anticancer Res. 1994 May-Jun;14(3A):841-6.
Mouse B16 melanoma cells (B16, parental line) and two derived clones either pigmented (B16P) or non pigmented (B16NP) were cultured as monolayers (2D) or on agar, as aggregates (3D). The productions of gelatinases A and B (72 kDa and 92 kDa type IV collagenases) and their inhibitors (TIMP1 and TIMP2), plasminogen activators (PAs) and plasminogen activator inhibitors (PAI) were investigated. The B16 cell lines did not secrete any gelatinase, but they secreted TIMP2, tissue-type (t-PA), urokinase-type (u-PA) plasminogen activators and PAI-1 like activities. High levels of PAI activity were determined in conditioned media and cellular extracts of B16NP, which could account for the lower tumorigenic potential of these cells. In 3D cultures, the cellular extracts of the three cell lines contained essentially u-PA activity. This activity could contribute to the greater tumorigenic and invasive capacities of B16, B16P and B16NP when cultured in 3D.
小鼠B16黑色素瘤细胞（B16，亲代细胞系）以及两个衍生克隆，一个有色素（B16P），另一个无色素（B16NP），分别以单层细胞（二维培养）或在琼脂上形成聚集体（三维培养）的方式进行培养。研究了明胶酶A和B（72 kDa和92 kDa的IV型胶原酶）及其抑制剂（TIMP1和TIMP2）、纤溶酶原激活剂（PAs）和纤溶酶原激活剂抑制剂（PAI）的产生情况。B16细胞系不分泌任何明胶酶，但它们分泌TIMP2、组织型（t-PA）、尿激酶型（u-PA）纤溶酶原激活剂以及PAI-1样活性物质。在B16NP的条件培养基和细胞提取物中检测到高水平的PAI活性，这可能解释了这些细胞较低的致瘤潜力。在三维培养中，这三种细胞系的细胞提取物主要含有u-PA活性。当在三维环境中培养时，这种活性可能有助于B16、B16P和B16NP具有更强的致瘤和侵袭能力。
