Githens S, Schexnayder J A, Frazier M L
Department of Biological Sciences, University of New Orleans, Louisiana.
Pancreas. 1992;7(5):556-61. doi: 10.1097/00006676-199209000-00008.
Our goal is to create a transgenic mouse model for human pancreatic duct cell adenocarcinoma using the promoter/enhancer region of the carbonic anhydrase (CA) II gene to drive the expression of SV-40 T-antigen in pancreatic duct cells. This requires that the CA II gene be expressed in mouse pancreatic duct cells and not in other pancreatic cells, as has already been shown to be the case in the human and guinea pig pancreas. We have shown with an enzyme histochemical assay that mouse pancreatic duct cells contain CA activity in both intact pancreas and cultured interlobular duct epithelium. In addition, CA activity was detected with a biochemical assay in homogenates of cultured duct epithelium. The specific activity of duct cells was 2.75-fold greater than in whole pancreas, suggesting that a substantial amount of total pancreatic CA activity is contributed by duct cells. At least some of the CA in cultured duct cells was inferred to be CA II by Northern blot analysis of RNA extracted from the cells. The concentration of CA II mRNA in the cultured duct cells was substantially greater than in whole pancreas and would appear to account for the majority, if not all, of the CA II in the mouse pancreas.
我们的目标是利用碳酸酐酶（CA）II基因的启动子/增强子区域，在胰腺导管细胞中驱动SV - 40 T抗原的表达，从而创建一种用于人类胰腺导管细胞腺癌的转基因小鼠模型。这要求CA II基因在小鼠胰腺导管细胞中表达，而不在其他胰腺细胞中表达，正如在人类和豚鼠胰腺中已被证实的那样。我们通过酶组织化学分析表明，在完整胰腺和培养的小叶间导管上皮中，小鼠胰腺导管细胞都含有CA活性。此外，在培养的导管上皮匀浆中通过生化分析检测到了CA活性。导管细胞的比活性比整个胰腺高2.75倍，这表明胰腺中相当一部分的总CA活性是由导管细胞贡献的。通过对从细胞中提取的RNA进行Northern印迹分析，推断培养的导管细胞中至少有一些CA是CA II。培养的导管细胞中CA II mRNA的浓度明显高于整个胰腺，似乎占小鼠胰腺中CA II的大部分，如果不是全部的话。
