Davis R E, Smoller B R
Department of Pathology, Stanford University Medical Center, California 94305.
Am J Pathol. 1992 Sep;141(3):691-8.
Lymphocytes in formalin-fixed skin biopsies from patients with cutaneous acute graft-versus-host disease (aGVHD) were studied with HECA-452 (an antibody recognizing lymphocytes with skin-homing properties) and a panel of antibodies recognizing pan-B (L26 [CD20]), pan-T (L60 [CD43] and A6 [CD45RA]), and T-helper subset (OPD4) antigens in paraffin sections. Biopsies from patients with erythema multiforme (EM) were similarly studied for comparison. In both conditions, T lymphocytes stained by OPD4 were predominantly confined to the dermis, whereas those stained by HECA-452 were concentrated in the epidermis; however, there was considerable variation between cases, and overlap between findings in the dermis and epidermis. Lymphocytes similarly studied in paraffin sections of liver, salivary gland, and gut affected by aGVHD were essentially unreactive with HECA-452, although they were largely stained by pan-T markers and showed some comparable reactivity with OPD4. The findings suggest that aGVHD of the skin is mediated by a different set of lymphocytes than in gut organs, and may have a similar immunologic mechanism to EM.
利用HECA - 452（一种识别具有皮肤归巢特性淋巴细胞的抗体）以及一组识别石蜡切片中泛B（L26 [CD20]）、泛T（L60 [CD43]和A6 [CD45RA]）和T辅助亚群（OPD4）抗原的抗体，对皮肤急性移植物抗宿主病（aGVHD）患者福尔马林固定皮肤活检组织中的淋巴细胞进行了研究。对多形红斑（EM）患者的活检组织进行了类似研究以作比较。在这两种情况下，OPD4染色的T淋巴细胞主要局限于真皮，而HECA - 452染色的淋巴细胞集中在表皮；然而，病例之间存在相当大的差异，且真皮和表皮的结果存在重叠。在受aGVHD影响的肝脏、唾液腺和肠道石蜡切片中同样研究的淋巴细胞，尽管它们大多被泛T标记物染色且与OPD4有一些类似的反应性，但基本上与HECA - 452无反应。这些发现表明，皮肤的aGVHD由与肠道器官不同的一组淋巴细胞介导，并且可能具有与EM相似的免疫机制。
