[Chemical-enzymatic synthesis and cloning in Escherichia coli of a gene coding for human granulocyte-colony stimulating factor].
作者信息
Kash'ian S K, Bystrov N S, Boldyreva E F, Poliakova I A, Severtsova I V, Korobko V G
出版信息
Bioorg Khim. 1992 Jan;18(1):71-7.
PMID:1381918
Abstract
Two artificial genes, encoding two forms of human granulocyte colony stimulating factor as products of a normal and an alternative splicing, have been by a chemical-enzymatic way synthesized and cloned in Escherichia coli. The genes are supplied with recognition sites of restriction endonucleases to facilitate the further cassette mutagenesis.
摘要
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