el-Meleigy M A
Department of Botany, Faculty of Science for Girls, Al-Azhar University, Madinet Nasr, Cairo, Egypt.
Biol Trace Elem Res. 1992 Aug;34(2):177-83. doi: 10.1007/BF02785245.
The presence of copper in the growth medium induces the biosynthesis of several low-mol-wt copper ogano-chelators in Aspergillus fumigatus and Penicillium chrysogenum. By use of P. chrysogenum, we were able to prepare several low-mol-wt metallothionein chelators, as well as several short-chain copper chelatins. The amino acid composition of a chelatin of mol wt approx 799 Da revealed the presence of cysteine, glutamic acid, glycine, and leucine. Use of A. fumigatus led to fewer copper chelators and one very short-chain peptide: a chelatin of mol wt approx 624 Da. composed of four amino acids, asparagine, glutamic acid, cysteine, and glycine. These results may confirm our assumption that fungi detoxify the deleterious action of toxic elements by producing high levels of heavy metal chelators/chelatins.
生长培养基中铜的存在会诱导烟曲霉和产黄青霉中几种低分子量铜有机螯合剂的生物合成。利用产黄青霉，我们能够制备几种低分子量金属硫蛋白螯合剂以及几种短链铜螯合素。一种分子量约为799 Da的螯合素的氨基酸组成显示含有半胱氨酸、谷氨酸、甘氨酸和亮氨酸。使用烟曲霉则产生较少的铜螯合剂和一种非常短链的肽：一种分子量约为624 Da的螯合素，由天冬酰胺、谷氨酸、半胱氨酸和甘氨酸四种氨基酸组成。这些结果可能证实了我们的假设，即真菌通过产生高水平的重金属螯合剂/螯合素来解毒有毒元素的有害作用。
