Abbass I M, Razak A A
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Biol Trace Elem Res. 1991 Mar;28(3):173-9. doi: 10.1007/BF02990464.
Aspergillus terreus was cultivated on Harrold's medium supplemented with 0.1% (w/v) cadmium chloride as well as on sulfur free medium amended with 0.1% (w/v) sodium selenite and potassium tellurite separately. The cell free extract of the fungus for each treatment was fractionated on a column packed with Sephadex G 75. The results demonstrated the ability of the fungus to synthesize several cadmium, selenium, and tellurium-binding proteins as well as metallothionein. The results suggested the biosynthesis of heavy metals chelators as well. The amino acids composition of a cadmium-binding metallothionein revealed the presence of high levels of both aromatic and sulfur amino acids in the hydrolysate.
土曲霉在添加了0.1%（w/v）氯化镉的哈罗德培养基上培养，同时也分别在添加了0.1%（w/v）亚硒酸钠和亚碲酸钾的无硫培养基上培养。对每种处理的真菌无细胞提取物在装有葡聚糖G 75的柱上进行分级分离。结果表明该真菌有能力合成几种镉、硒和碲结合蛋白以及金属硫蛋白。结果还表明了重金属螯合剂的生物合成。一种镉结合金属硫蛋白的氨基酸组成显示水解产物中存在高水平的芳香族和含硫氨基酸。
