Baudouin C, Brignole F, Fredj-Reygrobellet D, Nègre F, Bayle J, Gastaud P
Department of Ophthalmology, Saint-Roch Hospital, Nice, France.
Invest Ophthalmol Vis Sci. 1992 Sep;33(10):2822-9.
Immunotoxins directed against a membrane marker of cell proliferation, transferrin receptor, were investigated to inhibit the growth of retinal pigment epithelial (RPE) cells in proliferative vitreoretinopathy (PVR). We undertook an immunocytological study in specimens of vitreous, subretinal fluid, and epiretinal membranes from patients with PVR to address the expression of transferrin receptor by proliferating pigment epithelial cells during the course of PVR and in normal human ocular structures. Thirty four specimens of vitreous and subretinal fluid, as well as seven epiretinal membranes, were immunocytologically examined using monoclonal antibodies to transferrin receptor. They showed a strong expression of this marker by a large majority of the cells in these two periretinal fluids (mean percentages 80 and 91% in vitreous and subretinal fluid, respectively). In contrast, only a few cells within epiretinal membranes were found to express transferrin receptor. In normal human eye sections conjunctival and corneal epithelial cells, subcapsular epithelium of the lens strongly expressed transferrin receptor, whereas RPE cells remained negative to antitransferrin receptor antibodies. A few iris or ciliary pigment epithelial cells reacted weakly. Thus, this study shows that most intravitreal and subretinal fluid proliferating cells strongly express transferrin receptor on their surface. Also confirmed is that immunotoxins to this membrane antigen could constitute potentially useful therapeutic agents in PVR.
针对细胞增殖膜标志物转铁蛋白受体的免疫毒素被研究用于抑制增殖性玻璃体视网膜病变（PVR）中视网膜色素上皮（RPE）细胞的生长。我们对PVR患者的玻璃体、视网膜下液和视网膜前膜标本进行了免疫细胞学研究，以探讨在PVR过程中以及在正常人眼结构中增殖的色素上皮细胞中转铁蛋白受体的表达情况。使用抗转铁蛋白受体单克隆抗体对34份玻璃体和视网膜下液标本以及7份视网膜前膜进行了免疫细胞学检查。在这两种视网膜周围液中，绝大多数细胞强烈表达该标志物（玻璃体和视网膜下液中的平均百分比分别为80%和91%）。相比之下，仅发现视网膜前膜内有少数细胞表达转铁蛋白受体。在正常人眼切片中，结膜和角膜上皮细胞、晶状体囊下上皮强烈表达转铁蛋白受体，而RPE细胞对抗转铁蛋白受体抗体呈阴性。少数虹膜或睫状体色素上皮细胞反应较弱。因此，本研究表明，大多数玻璃体内和视网膜下液增殖细胞在其表面强烈表达转铁蛋白受体。还证实了针对这种膜抗原的免疫毒素可能构成PVR中潜在有用的治疗药物。
