使用复制平板试验快速评估唾液蛋白与细菌的相互作用。

Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions.

作者信息

Tseng C C, Scannapieco F A, Levine M J

机构信息

School of Dental Medicine, State University of New York at Buffalo.

出版信息

Oral Microbiol Immunol. 1992 Feb;7(1):53-6. doi: 10.1111/j.1399-302x.1992.tb00021.x.

DOI:10.1111/j.1399-302x.1992.tb00021.x

PMID:1382259

Abstract

A replica-plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep-blood (SB) agar were replica-plated onto nitrocellulose membranes overlaying SB or mitis-salivarius agar. Membranes with attached colonies were removed and incubated with 125I-amylase or 125I-proline-rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

摘要

采用影印平板试验筛选唾液分子与牙菌斑细菌之间的相互作用。将在羊血（SB）琼脂上培养的龈上菌斑中的细菌菌落影印接种到覆盖有SB或唾液变形链球菌琼脂的硝酸纤维素膜上。取下带有附着菌落的膜，与125I-淀粉酶或125I-富含脯氨酸糖蛋白（PRG）一起孵育。通过放射自显影检测阳性相互作用。只有戈登链球菌和粘性放线菌菌株结合淀粉酶，而粘性放线菌菌株结合PRG。结果表明，淀粉酶和PRG与需氧牙菌斑细菌的特定种类结合。