微粒体丙氨酸氨肽酶的免疫电子显微镜定位
Immunoelectron microscopic localization of microsomal alanine aminopeptidase.
作者信息
Kettmann U, Humbel B, Holzhausen H J, Bahn H, Aurich H
机构信息
Institute of Biochemistry, Faculty of Medicine, University of Halle, Germany.
出版信息
Acta Histochem. 1992;93(1):333-40. doi: 10.1016/S0065-1281(11)80233-3.
The localization of microsomal alanine aminopeptidase was investigated in the rat kidney. Resin embedding failed to demonstrate the localization of the enzyme by immunogold labelling. Using a cryo-ultramicrotomy method the enzyme could be detected on the luminal side of the brush border membrane of proximal tubular cells and to a lesser degree in their mitochondria. Furthermore, vesicular structures labelled with gold were found in the cytoplasma in the apical region of these cells.
对大鼠肾脏中的微粒体丙氨酸氨基肽酶进行了定位研究。树脂包埋未能通过免疫金标记显示该酶的定位。使用冷冻超薄切片法，可在近端肾小管细胞刷状缘膜的管腔侧检测到该酶，在其线粒体中也能检测到，但程度较轻。此外，在这些细胞顶端区域的细胞质中发现了用金标记的囊泡结构。
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