Kline R A, Negendank W, McCoy L E
Dept. of Surgery, Wayne State University, Detroit, MI 48201.
Biomater Artif Cells Immobilization Biotechnol. 1992;20(2-4):979-83. doi: 10.3109/10731199209119751.
A cat stroke model was used to evaluate the efficacy of Dextran-40 (DEX) or Fluosol-DA 20% (FDA) in the treatment of focal cerebral ischemia. The animals were assigned randomly to one of three treatment groups: control, isovolemic hemodilution with DEX or isovolemic hemodilution with FDA. The oxidation state of cytochrome aa3 was measured in-vivo using near infrared reflectance spectrophotometry. The cerebral edema was measured by magnetic resonance imaging (MRI). The MRI edema indices for the three groups revealed that the FDA group had less edema (p less than 0.05), approaching that of non-stroke controls. The relative oxidation state of aa3 for the DEX group declined both during and after hemodilution. At the ninth hour post stroke the FDA group was better (aa3 more oxidized. p less than 0.025). Changes in blood and plasma components were reflective of the extent of hemodilution. Whole blood viscosity analysis revealed a difference (p less than 0.05) at the lower shear rates comparing DEX to FDA with FDA being higher than DEX. Two animals in each of the groups were allowed to awaken at the end of the procedure for functional assessment. These observations suggest that hemodilution with FDA following stroke significantly reduces early post-ischemic cerebral edema, improves oxidation in the peri-infarct area and appears to minimize functional deficits.
使用猫中风模型来评估右旋糖酐-40（DEX）或20%氟碳乳剂（FDA）治疗局灶性脑缺血的疗效。将动物随机分为三个治疗组之一：对照组、用DEX进行等容血液稀释组或用FDA进行等容血液稀释组。使用近红外反射分光光度法在体内测量细胞色素aa3的氧化状态。通过磁共振成像（MRI）测量脑水肿。三组的MRI水肿指数显示，FDA组的水肿较少（p<0.05），接近非中风对照组。DEX组的aa3相对氧化状态在血液稀释期间和之后均下降。中风后第九小时，FDA组情况更好（aa3氧化程度更高，p<0.025）。血液和血浆成分的变化反映了血液稀释的程度。全血粘度分析显示，在较低剪切速率下，DEX与FDA相比存在差异（p<0.05），FDA高于DEX。每组中有两只动物在实验结束时苏醒以进行功能评估。这些观察结果表明，中风后用FDA进行血液稀释可显著减轻缺血后早期脑水肿，改善梗死周边区域的氧化状态，并似乎能使功能缺陷最小化。
