Kline R A, Negendank W, McCoy L, Berguer R
Department of Surgery, Wayne State University School of Medicine, Detroit, Michigan.
Am J Surg. 1991 Aug;162(2):103-6. doi: 10.1016/0002-9610(91)90168-d.
In a clinically applicable cat stroke model, 16 purpose-bred adult animals were used to evaluate the beneficial effects of two treatment regimens: isovolemic hemodilution with either a perfluorocarbon emulsion or dextran 40 (a glucose polymer). Animals that received these treatment regimens were then compared with a control group of untreated animals. Focal cerebral infarctions were produced by transorbital ligation of the left middle cerebral artery. The randomly allocated treatment arms of the study were instituted 3 hours after ligation of the middle cerebral artery, thereby simulating a human clinical situation. In vivo mitochondrial metabolic activity of the peri-infarct cerebral tissue was continually assessed by means of a multiwavelength near-infrared spectrophotometer. This allowed measurement of cellular oxygenation at the cytochrome aa3 level, the terminal member of the cytochrome chain. Sequential proton-based magnetic resonance imaging was used to measure intracerebral water in vivo. Cardiac output, oxygen consumption/delivery, chemical, histologic, and rheologic parameters were also assessed. The data collected were analyzed by group means and standard statistical analyses, which revealed that the group treated with the perfluorocarbon emulsion had both less brain edema in the early post-infarct period (p less than 0.05), as well as a higher level of oxidation of cytochrome aa3 (p less than or equal to 0.025). This evidence supports the premise that isovolemic hemodilution with an oxygen-carrying hemodiluent may be beneficial in the treatment of ischemic strokes.
在一种临床适用的猫中风模型中，使用16只专门培育的成年动物来评估两种治疗方案的有益效果：用全氟碳乳剂或右旋糖酐40（一种葡萄糖聚合物）进行等容血液稀释。然后将接受这些治疗方案的动物与未治疗的动物对照组进行比较。通过经眶结扎左大脑中动脉产生局灶性脑梗死。在大脑中动脉结扎3小时后开始该研究的随机分配治疗组，从而模拟人类临床情况。通过多波长近红外分光光度计持续评估梗死灶周围脑组织的体内线粒体代谢活性。这使得能够在细胞色素aa3水平（细胞色素链的末端成员）测量细胞氧合。使用基于质子的序列磁共振成像在体内测量脑内水分。还评估了心输出量、氧消耗/输送、化学、组织学和流变学参数。收集的数据通过组均值和标准统计分析进行分析，结果显示用全氟碳乳剂治疗的组在梗死后期早期脑水肿较少（p小于0.05），并且细胞色素aa3的氧化水平较高（p小于或等于0.025）。这一证据支持了用携氧血液稀释剂进行等容血液稀释可能对缺血性中风治疗有益的前提。
