Glasky M S, Yin A, Smith L H, Bieber M, Teng N N
Department of Gynecology and Obstetrics, Stanford University of Medicine, CA 94305.
Hum Antibodies Hybridomas. 1992 Jul;3(3):114-22.
A human monoclonal antibody (MAb), MS2B6, produced from splenocytes isolated from a patient with advanced papillary serous cystadenocarcinoma of the ovary, defines a unique human tumor-associated antigen. This antigen, EA2B6 (epithelial antigen 2B6), is expressed in a tissue-restricted manner on cultured and fresh human adenocarcinomas and some normal glandular epithelial tissues. EA2B6 is a 38-48 kD protein antigen that co-fractionates with the nuclear matrix-intermediate filament scaffold of simple glandular epithelial tissues. EA2B6 is a molecule with restricted solubility, and in vitro antigen-antibody binding is dependent on the antigen being presented on a solid support. To determine if EA2B6 is a cytokeratin, competition studies were undertaken with several cytokeratin-specific murine monoclonal antibodies. None of these antibodies inhibited the binding of human MAb MS2B6 to partially purified EA2B6. Less than 1% of HT29 colon adenocarcinoma cells and fresh ovarian adenocarcinoma ascites cells express EA2B6 on their surface. The majority of EA2B6 is intracellular. Because of the restricted tissue distribution of this antigen and stability of the antibody, we believe MS2B6 is a good candidate for MAb-mediated diagnosis and therapy of human adenocarcinomas.
一种人单克隆抗体（MAb）MS2B6，由从一名晚期卵巢乳头状浆液性囊腺癌患者分离的脾细胞产生，它定义了一种独特的人肿瘤相关抗原。这种抗原，EA2B6（上皮抗原2B6），在培养的和新鲜的人腺癌以及一些正常腺上皮组织中以组织限制性方式表达。EA2B6是一种38 - 48 kD的蛋白质抗原，与简单腺上皮组织的核基质 - 中间丝支架共分离。EA2B6是一种溶解性受限的分子，体外抗原 - 抗体结合依赖于抗原呈现在固体支持物上。为了确定EA2B6是否为细胞角蛋白，用几种细胞角蛋白特异性鼠单克隆抗体进行了竞争研究。这些抗体均未抑制人MAb MS2B6与部分纯化的EA2B6的结合。不到1%的HT29结肠腺癌细胞和新鲜卵巢腺癌腹水细胞在其表面表达EA2B6。大多数EA2B6位于细胞内。由于这种抗原的组织分布受限以及抗体的稳定性，我们认为MS2B6是用于人腺癌的单克隆抗体介导的诊断和治疗的良好候选物。
