Smith L H, Yin A, Glasky M S, Tyler N, Robles M, Foster C A, Bieber M, Teng N N
Department of Obstetrics and Gynecology, University of California-Davis School of Medicine, Sacramento 95816.
Am J Obstet Gynecol. 1992 Feb;166(2):634-45. doi: 10.1016/0002-9378(92)91690-c.
MS2B6, a human monoclonal antibody derived from a patient with advanced ovarian cancer, has been used to study the distribution and characteristics of its target antigen. The MS2B6 antigen was detected by immunoperoxidase studies in 41 of 41 epithelial ovarian cancers and in the majority of nonovarian adenocarcinomas. Among normal tissues the MS2B6 antigen was found in the adult epithelia of the fallopian tube, endometrium, endocervix, colon, bronchus, breast, sweat duct, and large renal ducts. No detectable antigen was found in peritoneal epithelia, tissue stromal cells, spleen, thymus, or blood-borne cells. Immunoblotting analysis showed that the MS2B6 epitope resides on polypeptides of 38, 44, and 60 kd. The cellular location of the MS2B6 antigen was studied with immunoperoxidase and immunofluorescent staining and immunoelectronmicroscopy of ovarian cancer ascites tumor cells. The results suggest that in ascites tumor cells the MS2B6 antigen is located in a layer of the peripheral cytoplasm beginning just below the cell membrane. MS2B6 may be useful as an imaging or therapeutic agent.
MS2B6是一种源自晚期卵巢癌患者的人单克隆抗体，已被用于研究其靶抗原的分布和特性。通过免疫过氧化物酶研究在41例上皮性卵巢癌中的41例以及大多数非卵巢腺癌中检测到了MS2B6抗原。在正常组织中，MS2B6抗原存在于输卵管、子宫内膜、子宫颈内膜、结肠、支气管、乳腺、汗腺导管和大肾导管的成人上皮中。在腹膜上皮、组织基质细胞、脾脏、胸腺或血源性细胞中未发现可检测到的抗原。免疫印迹分析表明，MS2B6表位存在于38、44和60 kDa的多肽上。利用免疫过氧化物酶、免疫荧光染色以及卵巢癌腹水肿瘤细胞的免疫电子显微镜技术研究了MS2B6抗原的细胞定位。结果表明，在腹水肿瘤细胞中，MS2B6抗原位于刚好在细胞膜下方开始的一层外周细胞质中。MS2B6可用作成像或治疗剂。
