Fernández-López J A, Casado J, Argilés J M, Alemany M
Department de Bioquímica i Fisiologia, Universitat de Barcelona, Spain.
Arch Int Physiol Biochim Biophys. 1992 May-Jun;100(3):231-6. doi: 10.3109/13813459208998106.
Intestinal lymph flow in rats receiving an oral load of 3 mmoles of glucose was determined by measuring the amount of tritiated gavage water entering via the portal route [computed differential arterio-venous tritium water concentrations multiplied by portal blood flow) and measuring the total amount of tritium transferred from the intestinal lumen to the rest of the rat in a given time [from the blood tritium specific activity and the water space (795 ml.kg-1) of the rat]. The approximate figure obtained (79.8 ml.kg-1.h) was used for the evaluation of the amount of glucose (from 14C-labelled glucose in the gavage) transfer via intestinal lymph from the label present in the lymph and its flow. Lymph glucose concentration (c. 11 mM) and specific radioactivity were higher than those of blood. The amount of glucose actually taken up from the intestinal lumen was determined measuring the remaining label in the alimentary canal. The portal contribution to label distribution was measured as for tritium water transfer. Most glucose label passed through in the form of glucose, with much smaller proportions as CO2 and even smaller as lactate and other labelled compounds. The results suggest that the contribution of lymph circulation to dietary glucose incorporation into the bloodstream may be significant, amounting to values higher than 10% of the glucose actually incorporated.
通过测量经门静脉途径进入的氚标记灌胃水的量（计算动脉-静脉氚水浓度差乘以门静脉血流量），并测量在给定时间内从肠腔转移到大鼠其余部分的氚的总量（根据大鼠血液中的氚比活度和水空间（795 ml·kg⁻¹）），来测定口服3毫摩尔葡萄糖的大鼠的肠淋巴流量。所获得的近似数值（79.8 ml·kg⁻¹·h）用于评估葡萄糖（来自灌胃中的¹⁴C标记葡萄糖）通过肠淋巴从淋巴中存在的标记物及其流量的转移量。淋巴葡萄糖浓度（约11 mM）和比放射性高于血液中的浓度。通过测量消化道中剩余的标记物来确定实际从肠腔摄取的葡萄糖量。门静脉对标记物分布的贡献测量方法与氚水转移相同。大多数葡萄糖标记物以葡萄糖形式通过，以二氧化碳形式通过的比例小得多，以乳酸和其他标记化合物形式通过的比例更小。结果表明，淋巴循环对膳食葡萄糖进入血液循环的贡献可能很大，高达实际摄入葡萄糖的10%以上。
