Dalle M, Dauprat-Dalle P, Barlet J P
Laboratoire de Physiologie Animale, Université Blaise Pascal, Aubière, France.
Arch Int Physiol Biochim Biophys. 1992 May-Jun;100(3):251-4. doi: 10.3109/13813459208998110.
Synthetic human parathyroid hormone-related peptide (hPTHrP)-(1-34) fragment was compared with parathyroid hormone (bovine sequence, 1-34; bPTH-(1-34) for inhibiting oxytocin or prostaglandin F2 alpha (PGF2 alpha)-induced contractions on rat uterus in vitro. bPTH exhibited a potent (ED50 = 7 x 10(-9) M) inhibition on oxytocin-induced contractions. Both bPTH-(1-34) and hPTHrP-(1-34) were devoided of any significant effect upon PGF2 alpha-induced uterine contractions. Human PTHrP also inhibited oxytocin-induced uterine contractions (ED50 = 77 x 10(-9) M) and this effect, like that of bPTH, was dose dependent. Human PTHrP-(140-173) fragment had no significant effect on oxytocin-induced uterine contractions. The inhibitory effect of hPTHrP-(1-34) disappeared after pretreatment with [Tyr]34-bPTH-(7-34)-NH2, a competitive reversible antagonist of bPTH-(1-34). Thus PTHrP might be involved in the control of myometrial activity.
将合成的人甲状旁腺激素相关肽（hPTHrP）-（1-34）片段与甲状旁腺激素（牛序列，1-34；bPTH-（1-34））进行比较，以研究其对大鼠子宫体外催产素或前列腺素F2α（PGF2α）诱导的收缩的抑制作用。bPTH对催产素诱导的收缩表现出强效抑制作用（ED50 = 7×10^(-9) M）。bPTH-（1-34）和hPTHrP-（1-34）对PGF2α诱导的子宫收缩均无显著影响。人PTHrP也抑制催产素诱导的子宫收缩（ED50 = 77×10^(-9) M），且这种作用与bPTH一样，呈剂量依赖性。人PTHrP-（140-173）片段对催产素诱导的子宫收缩无显著影响。用bPTH-（1-34）的竞争性可逆拮抗剂[Tyr]34-bPTH-（7-34）-NH2预处理后，hPTHrP-（1-34）的抑制作用消失。因此，PTHrP可能参与子宫肌层活动的调控。
