Shew R L, Yee J A, Pang P K
J Pharmacol Exp Ther. 1984 Jul;230(1):1-6.
Synthetic bovine parathyroid hormone containing the 1-34 NH2 terminal amino acids [bPTH-(1-34)] is capable of inhibiting stimulated uterine contraction. The purpose of the present investigation is to determine whether the inhibitory action of bPTH-(1-34) is a direct action of the hormone fragment. The effect of different synthetic preparations of bPTH-(1-34), salmon calcitonin, corticotropin-inhibiting peptide and bovine serum albumin on oxytocin-stimulated uterine contraction was determined. In addition, the effects of atropine, propranolol, phentolamine, pyrilamine, cimetidine and the prostaglandin synthetase inhibitor indomethacin on the inhibitory action of bPTH-(1-34) on uterine contraction was determined. Both synthetic preparations of bPTH-(1-34) inhibited oxytocin-initiated contractions similarly. Salmon calcitonin, corticotropin-inhibiting peptide, and bovine serum albumin did not alter oxytocin-stimulated uterine contractions. The salmon calcitonin also did not alter the ability of bPTH-(1-34) to exert its inhibitory effect on uterine contraction. Cholinergic, alpha and beta adrenergic, histaminergic (H1 and H2) and prostaglandin synthetase inhibitors did not alter the action of bPTH-(1-34). These results suggest that the action of bPTH-(1-34) is 1) not due to the presence of a contaminant in the synthetic hormone preparation and 2) that the effect could be due to a direct action effect of the hormone fragment on uterine tissue.
含1 - 34个氨基末端氨基酸的合成牛甲状旁腺激素[bPTH-(1 - 34)]能够抑制刺激引起的子宫收缩。本研究的目的是确定bPTH-(1 - 34)的抑制作用是否是该激素片段的直接作用。测定了bPTH-(1 - 34)的不同合成制剂、鲑鱼降钙素、促肾上腺皮质激素抑制肽和牛血清白蛋白对催产素刺激的子宫收缩的影响。此外，还测定了阿托品、普萘洛尔、酚妥拉明、吡苄明、西咪替丁和前列腺素合成酶抑制剂吲哚美辛对bPTH-(1 - 34)子宫收缩抑制作用的影响。bPTH-(1 - 34)的两种合成制剂对催产素引发的收缩的抑制作用相似。鲑鱼降钙素、促肾上腺皮质激素抑制肽和牛血清白蛋白未改变催产素刺激的子宫收缩。鲑鱼降钙素也未改变bPTH-(1 - 34)对子宫收缩发挥抑制作用的能力。胆碱能、α和β肾上腺素能、组胺能（H1和H2）和前列腺素合成酶抑制剂均未改变bPTH-(1 - 34)的作用。这些结果表明，bPTH-(1 - 34)的作用：1）不是由于合成激素制剂中存在污染物；2）该作用可能是由于激素片段对子宫组织的直接作用。
